Ministry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau & Inner Mongolia, Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, School of Ecology and Environment, Inner Mongolia University, Hohhot 010040, China.
Ministry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau & Inner Mongolia, Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, School of Ecology and Environment, Inner Mongolia University, Hohhot 010040, China; Inner Mongolia Lvchuang Environmental Protection Technology Co., Ltd., Hohhot 010051, China.
Bioresour Technol. 2023 Feb;370:128565. doi: 10.1016/j.biortech.2022.128565. Epub 2022 Dec 31.
The purpose of this study was to explore the tolerance mechanism of anammox consortia in microbial fuel cell (MFC) system at low temperature. Data showed that nearly 80 % total nitrogen removal was achieved after the temperature decreased from 30 °C to 15 °C. The nitrogenremovalrate (NRR) of the system was decreased by 26.3 %, from 0.441 kgN·m·d at 30 °C to 0.325 kgN·m·d at 15 °C. Isotope experiment in NH-containing reactor found that much more N were produced than N, confirming that anammox was the main NH removal pathway and electrochemical oxidation participate in this process. High throughput sequencing analysis indicated the low temperature stimulated the enrichment of heterotrophic bacteria, such as Comamonadaceae and Rhodobacteraceae. While the relative abundance of Candidatus Brocadia, typical anammox bacteria, decreased significantly. Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis showed that the low temperature induced a more efficient expression in synthesis of unsaturated fatty acids (UFAs) and ABC membrane transports. This study indicates that anammox consortia are likely to maintain high nitrogen removal performance of MFC system by changing the proportion of membrane composition and EPS exportation.
本研究旨在探索氨氮单胞菌（Anammox）菌群在微生物燃料电池（MFC）系统中低温下的耐受机制。数据表明，当温度从 30°C 降至 15°C 时，近 80%的总氮去除率得以实现。系统的氮去除率（NRR）下降了 26.3%，从 30°C 时的 0.441 kgN·m·d 降至 15°C 时的 0.325 kgN·m·d。含 NH 的反应器中的同位素实验发现，产生的 N 比 N 多得多，这证实了 Anammox 是主要的 NH 去除途径，电化学氧化参与了这一过程。高通量测序分析表明，低温刺激了异养菌（如 Comamonadaceae 和 Rhodobacteraceae）的富集。而典型的 Anammox 菌 Candidatus Brocadia 的相对丰度则显著下降。京都基因与基因组百科全书（KEGG）途径分析表明，低温诱导了不饱和脂肪酸（UFAs）和 ABC 膜转运的合成更有效的表达。本研究表明，Anammox 菌群可能通过改变膜组成和 EPS 外排的比例来维持 MFC 系统的高氮去除性能。
