Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University.
Department of Neuroscience and Pathobiology, Research Institute of Environmental Medicine, Nagoya University.
J Nutr Sci Vitaminol (Tokyo). 2022;68(6):481-487. doi: 10.3177/jnsv.68.481.
We previously demonstrated that ascorbic acid (AsA) deficiency, caused by an AsA-free diet, induces inflammatory changes in the liver and intestine of osteogenic disorder Shionogi (ODS) rats that cannot synthesize AsA. However, whether low AsA intake induces inflammatory changes remains unknown. Here, we assessed the inflammatory changes in ODS rats caused by low AsA intake and compared them to ODS rats that were fed a diet supplemented with sufficient amounts of AsA (300 mg/kg). Male ODS rats (12-wk-old) were fed an AsA-free diet (0 ppm group), AsA 20 mg/kg diet (20 ppm group), AsA 40 mg/kg diet (40 ppm group) or AsA 300 mg/kg diet (300 ppm group) for 22 d. The hepatic mRNA levels of acute phase proteins, including C-reactive protein (CRP) and haptoglobin, were higher in the 0 and 20 ppm groups, than in the 300 and 40 ppm groups, but were not significantly higher in the 20 ppm group. Serum CRP concentrations were significantly higher in the 0 and 20 ppm groups than in the 300 and 40 ppm groups. Jejunal and ileal interleukin-1β (IL-1β) mRNA levels were higher in the 0 and 20 ppm groups than in the 300 ppm group. Jejunal and ileal IL-6 mRNA levels tended to be higher in the 0 and 20 ppm groups than in the 300 ppm group. Furthermore, the portal IL-6 concentration gradually increased with decrease in the AsA intake. Thus, inflammatory changes could occur in both AsA-deficient ODS rats and ODS rats with low AsA intake.
我们之前的研究表明，由于缺乏抗坏血酸（AsA）而导致的饮食中缺乏抗坏血酸（AsA）会引起不能合成 AsA 的成骨障碍 Shionogi（ODS）大鼠的肝脏和肠道发生炎症变化。然而，低摄入量的 AsA 是否会引起炎症变化尚不清楚。在这里，我们评估了低摄入量的 AsA 对 ODS 大鼠引起的炎症变化，并将其与喂食补充足够量 AsA（300mg/kg）的 ODS 大鼠进行了比较。12 周龄雄性 ODS 大鼠（12 周龄）喂食无 AsA 饮食（0ppm 组）、AsA 20mg/kg 饮食（20ppm 组）、AsA 40mg/kg 饮食（40ppm 组）或 AsA 300mg/kg 饮食（300ppm 组）22d。0 和 20ppm 组肝组织急性时相蛋白，包括 C 反应蛋白（CRP）和触珠蛋白，的 mRNA 水平高于 300 和 40ppm 组，但 20ppm 组无明显升高。0 和 20ppm 组血清 CRP 浓度显著高于 300 和 40ppm 组。0 和 20ppm 组空肠和回肠白细胞介素-1β（IL-1β）mRNA 水平高于 300ppm 组。0 和 20ppm 组空肠和回肠白细胞介素-6（IL-6）mRNA 水平呈升高趋势。此外，随着 AsA 摄入量的减少，门静脉 IL-6 浓度逐渐升高。因此，炎症变化可能发生在缺乏 AsA 的 ODS 大鼠和 AsA 摄入不足的 ODS 大鼠中。
