Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai, 602 105, India.
Department of Chemical Engineering, Hindusthan College of Engineering and Technology, Coimbatore, 641032, India.
Environ Sci Pollut Res Int. 2023 Apr;30(17):48948-48961. doi: 10.1007/s11356-022-24996-1. Epub 2023 Jan 5.
Biosorption is a key phenomenon that has been used to remove the aquatic pollutants like dyes and heavy metals present in industrial effluents. The current study aims at the development of a chitosan-pectinase blend (CPB) to separate the methylene blue (MB) dye from its synthetic solution. Pectinase, an enzyme isolated from a consortium of Bacillus species, is imbibed in the blend. The electron micrographs revealed the rough surface of the adsorbent, and its amorphous nature was evident from broader peaks in diffraction patterns. The FTIR analyses indicated the perfect blend formation through the presence and shifts in the characteristic peaks. Maximum adsorption was exhibited at pH 7.0, 30 °C, 30 min of contact time and an adsorbent dosage of 2.5 g/L. On comparison, the pseudo-second-order model was found to be the suitable fit with the highest R value closer to 1. Different isotherm models were experimentally fitted and the maximum adsorption capacity was obtained at 16.81 mg/g and the Temkin isotherm suits the best. The polymer blend showed an agreeable extent of desorption of MB dye which was evident from the desorption studies and, thereby, the biosorbent could be reused for removing the dye to the maximum extent.
生物吸附是一种关键现象，已被用于去除工业废水中存在的水溶性污染物，如染料和重金属。本研究旨在开发一种壳聚糖-果胶酶混合物（CPB），以从合成溶液中分离亚甲基蓝（MB）染料。果胶酶是从芽孢杆菌属的混合物中分离出来的一种酶，被吸收在混合物中。电子显微镜照片显示了吸附剂的粗糙表面，其无定形性质在衍射图谱中的宽峰中显而易见。FTIR 分析表明，通过存在和特征峰的移动，形成了完美的混合物。最大吸附在 pH 7.0、30°C、接触时间 30 分钟和吸附剂用量 2.5g/L 时达到。相比之下，发现伪二阶模型最适合，最高 R 值更接近 1。实验拟合了不同的等温线模型，最大吸附容量为 16.81mg/g，最适合的是 Temkin 等温线。聚合物混合物显示出对 MB 染料的可接受的解吸程度，这从解吸研究中可以明显看出，因此，生物吸附剂可以被重复使用以最大程度地去除染料。
