Liaocheng, Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng, 252059, China.
BMC Genom Data. 2023 Jan 4;24(1):2. doi: 10.1186/s12863-022-01101-6.
Previous studies have shown that the protein kinase cGMP-dependent 2 (PRKG2) gene is associated with dwarfism in humans, dogo Argentines, and Angus cattle, as well as with height and osteoblastogenesis in humans. Therefore, the PRKG2 gene was used as the target gene to explore whether this gene is associated with several thoracolumbar vertebrae and carcass traits in Dezhou donkeys.
In this study, fifteen SNPs were identified by targeted sequencing, all of which were located in introns of the PRKG2 gene. Association analysis illustrated that the g.162153251 G > A, g.162156524 C > T, g.162158453 C > T and, g.162163775 T > G were significantly different from carcass weight. g.162166224 G > A, g.162166654 T > A, g.162167165 C > A, g.162167314 A > C and, g.162172653 G > C were significantly associated with the number of thoracic vertebrae. g.162140112 A > G was significantly associated with the number and the length of lumbar vertebrae, and g.162163775 T > G was significantly associated with the total number of thoracolumbar vertebrae.
Overall, the results of this study suggest that PRKG2 gene polymorphism can be used as a molecular marker to breed high-quality Dezhou donkeys.
先前的研究表明，蛋白激酶 cGMP 依赖性 2（PRKG2）基因与人、阿根廷杜高犬和安格斯牛的矮小症有关，也与人的身高和成骨细胞生成有关。因此，PRKG2 基因被用作目标基因，以探讨该基因是否与德州驴的几个胸腰椎和胴体性状有关。
在本研究中，通过靶向测序鉴定了 15 个 SNP，它们均位于 PRKG2 基因的内含子中。关联分析表明，g.162153251G>A、g.162156524C>T、g.162158453C>T和 g.162163775T>G 与胴体重显著不同。g.162166224G>A、g.162166654T>A、g.162167165C>A、g.162167314A>C和 g.162172653G>C 与胸椎数显著相关。g.162140112A>G 与腰椎数和长度显著相关，g.162163775T>G 与胸腰椎总数显著相关。
总的来说，本研究结果表明，PRKG2 基因多态性可作为分子标记用于德州驴的优质选育。
