De Stefano V, Leone G, De Carolis M P, Ferrelli R, De Carolis S, Pagano L, Tortorolo G, Bizzi B
Istituto di Semeiotica Medica, Università Cattolica, Roma, Italia.
Thromb Haemost. 1987 Jun 3;57(3):329-31.
Antithrombin III (AT III) plasma levels were investigated in 18 full term neonates and 14 healthy preterm neonates. A control group of 20 healthy adults was also studied. AT III was measured as antigen concentration (Ag) and antithrombin or anti-factor Xa heparin cofactor (H.C.) activities. Crossed immunoelectrophoresis on heparin-agarose (H-CIE) was carried out on plasma samples; moreover the distribution of isoantithrombins was investigated on whole plasma by a technique of crossed immunoelectrofocusing (CIEF). AT III plasma levels in full term infants were significantly lower as compared to the adult values. The preterm newborns group showed a further significant decrease in AT III levels as compared to the full term neonates. In all infants AT III H-CIE runs displayed a single fast moving anodal peak, so that a normal binding to heparin was demonstrated. The CIEF AT III plasma pattern of the adults as well as of all neonates displayed three major peaks at pH range 5.2-4.9, a small amount of AT III at pH 4.9-4.8 and a minor peak at pH 4.8-4.6, so that it was concluded that the isoantithrombins plasma distribution in neonatal age is identical to that of the adult subjects. Four neonates whose mothers were affected by AT III congenital defect were also investigated: diagnosis of congenital deficiency was established in three cases.
对18名足月新生儿和14名健康早产儿的抗凝血酶III（AT III）血浆水平进行了研究。还对20名健康成年人组成的对照组进行了研究。AT III通过抗原浓度（Ag）以及抗凝血酶或抗因子Xa肝素辅因子（H.C.）活性进行测量。对血浆样本进行肝素 - 琼脂糖交叉免疫电泳（H - CIE）；此外，通过交叉免疫电聚焦（CIEF）技术对全血中的异抗凝血酶分布进行了研究。足月婴儿的AT III血浆水平与成人相比显著降低。与足月新生儿相比，早产新生儿组的AT III水平进一步显著降低。在所有婴儿中，AT III H - CIE电泳显示出一个单一的快速移动阳极峰，表明与肝素的结合正常。成人以及所有新生儿的CIEF AT III血浆图谱在pH范围5.2 - 4.9处显示出三个主要峰，在pH 4.9 - 4.8处有少量AT III，在pH 4.8 - 4.6处有一个较小的峰，因此得出结论，新生儿期异抗凝血酶的血浆分布与成人相同。还对4名母亲患有AT III先天性缺陷的新生儿进行了研究：其中3例确诊为先天性缺乏。
