Shaoxing University School of Medicine, Shaoxing, Zhejiang Province 312000, China.
Department of Thoracic Surgery, The First Affiliated Hospital of Shaoxing University (Shaoxing People's Hospital), Shaoxing, Zhejiang Province 312000, China.
Mutat Res. 2023 Jan-Jun;826:111811. doi: 10.1016/j.mrfmmm.2022.111811. Epub 2022 Dec 15.
Searching for differential genes in lung adenocarcinoma (LUAD) is vital for research. Hyaluronan mediated motility receptor (HMMR) promotes malignant progression of cancer patients. However, the molecular regulators of HMMR-mediated LUAD onset are unknown. This work aimed to study the relevance of HMMR to proliferation, migration and invasion of LUAD cells. Let-7c-5p and HMMR levels in LUAD cells and HLF-a cells were assessed, and their correlation was also detected. Their interaction was determined by dual-luciferase experiments and qRT-PCR. Cell proliferation, migration and invasion potentials in vitro were validated through cell counting kit-8 (CCK-8), colony formation, scratch healing, and transwell assays. The expression of HMMR was examined by qRT-PCR and western blot and the expression of let-7c-5p was assayed by qRT-PCR. It was found that HMMR level was increased in LUAD and negatively correlated with let-7c-5p level. Let-7c-5p directly targeted HMMR to repress LUAD cell proliferation, migration and invasion. The above data illustrated that the let-7c-5p/HMMR axis may provide certain therapeutic value for LUAD patients.
寻找肺腺癌 (LUAD) 中的差异基因对于研究至关重要。透明质酸介导的运动受体 (HMMR) 促进了癌症患者的恶性进展。然而，HMMR 介导的 LUAD 发病的分子调节剂尚不清楚。本研究旨在研究 HMMR 与 LUAD 细胞增殖、迁移和侵袭的相关性。评估了 LUAD 细胞和 HLFa 细胞中的 Let-7c-5p 和 HMMR 水平，并检测了它们之间的相关性。通过双荧光素酶实验和 qRT-PCR 确定了它们的相互作用。通过细胞计数试剂盒-8 (CCK-8)、集落形成、划痕愈合和 Transwell 分析验证了体外细胞增殖、迁移和侵袭潜能。通过 qRT-PCR 和 Western blot 检测了 HMMR 的表达，通过 qRT-PCR 检测了 let-7c-5p 的表达。结果发现，HMMR 水平在 LUAD 中升高，与 let-7c-5p 水平呈负相关。Let-7c-5p 直接靶向 HMMR 抑制 LUAD 细胞增殖、迁移和侵袭。上述数据表明，let-7c-5p/HMMR 轴可能为 LUAD 患者提供一定的治疗价值。
