Antonov Yurij A, Zhuravleva Irina L, Bezrodnykh Evgeniya A, Berezin Boris B, Kulikov Sergey N, Tikhonov Vladimir E
N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
A. N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, Russia.
Carbohydr Polym. 2023 Feb 15;302:120391. doi: 10.1016/j.carbpol.2022.120391. Epub 2022 Nov 25.
Сomplexation of oligochitosan (OCHI) having the degree of acetylation (DA 26 %) with sodium caseinate (SC) at pH 5.8 and 7.2 is described and compared with the complexation of OCHI (DA 2 %) at pH 5.8. In the alkalescent medium, the complexation of OCHI (DA 26 %) is weaker and dualistic depending on SC concentration in the system. In the diluted alkalescent system, the formation of only soluble complexes is observed at OCHI/SC ratio ≤0.9. In the semi diluted one, the complexation results in the formation of insoluble complexes those composition changes symbatically with the OCHI/SC ratio in the system. At pH 5.8, OCHI/SC ratio in insoluble complexes remains the same regardless of OCHI/SC ratio in the solution. At pH 5.8, the electrostatic complexation weakens with an increase in DA and is completely suppressed at a high ionic strength. These results can be promising for construction of biodegradable protein/chitosan drug delivery systems.
描述了在pH 5.8和7.2条件下，乙酰化度为26%的低聚壳聚糖（OCHI）与酪蛋白酸钠（SC）的络合情况，并与pH 5.8条件下乙酰化度为2%的OCHI的络合情况进行了比较。在碱性介质中，OCHI（乙酰化度26%）的络合较弱，且根据体系中SC浓度的不同呈现二元性。在稀释的碱性体系中，当OCHI/SC比例≤0.9时，仅观察到可溶性络合物的形成。在半稀释体系中，络合导致形成不溶性络合物，其组成随体系中OCHI/SC比例呈对称变化。在pH 5.8时，不溶性络合物中的OCHI/SC比例与溶液中的OCHI/SC比例无关，保持不变。在pH 5.8时，静电络合随着乙酰化度的增加而减弱，并在高离子强度下被完全抑制。这些结果对于构建可生物降解的蛋白质/壳聚糖药物递送系统可能具有重要意义。
