Schmeer N, Krauss H, Apel J, Adami M, Müller H P, Schneider W, Perez-Martinez J A, Rieser H
Institute of Hygiene and Infectious Diseases of Animals, Justus-Liebig-University Giessen, F.R.G.
Vet Microbiol. 1987 Jun;14(2):125-35. doi: 10.1016/0378-1135(87)90004-6.
Enzyme-linked immunosorbent assays (ELISAs) specific for caprine IgG(H+L), IgG1 and IgG2 were developed and evaluated for serodiagnosis of Chlamydia psittaci infections in a Tunisian goat flock with currently occurring chlamydial abortions and a clinically inapparent goat flock of an animal research facility. Additionally, ELISAs were applied to record the IgG1 and IgG2 dynamics of four goats vaccinated with inactivated Chlamydia psittaci and Coxiella burnetii. For screening purposes, the IgG(H+L) ELISA proved to be superior to the complement fixation test because it detected a larger number of chlamydial abortions and was easier to perform and to interpret. Analysis of Chlamydia psittaci-specific IgG1 and IgG2 responses to naturally occurring infections by ELISA revealed high IgG1 levels associated with IgG2 in goats with current abortions, whereas clinically inapparent, but seropositive goats were characterized by significantly lower IgG1 levels only (P less than 0.001). Similarly, the four vaccinated goats responded initially with Chlamydia psittaci-specific IgG1, whereas second and third vaccinations induced (as in goats with chlamydial abortions) predominantly IgG1, but also IgG2. The results indicated that clinically inapparent chlamydial infection may be distinguished from overt disease by analysing specific IgG1 and IgG2 responses. Applying Coxiella burnetii- specific ELISAs on field samples, IgG1 alone could be detected in eight, IgG2 alone in one and IgG1 combined with IgG2 in nine goats. The coxiella-specific antibody response of the four vaccinated goats was--in contrast to the chlamydia-specific response--characterized by IgG2 dominance.
针对山羊IgG（H + L）、IgG1和IgG2开发了酶联免疫吸附测定（ELISA），并对突尼斯一个目前发生衣原体流产的山羊群以及一个动物研究设施中临床无症状的山羊群进行鹦鹉热衣原体感染的血清学诊断评估。此外，还应用ELISA记录了四只接种灭活鹦鹉热衣原体和伯氏考克斯体的山羊的IgG1和IgG2动态变化。为了进行筛查，IgG（H + L）ELISA被证明优于补体结合试验，因为它检测到更多的衣原体流产病例，且操作和解读更容易。通过ELISA分析鹦鹉热衣原体特异性IgG1和IgG2对自然感染的反应发现，当前流产的山羊中，IgG1水平高且与IgG2相关，而临床无症状但血清学阳性的山羊仅表现为IgG1水平显著较低（P小于0.001）。同样，四只接种疫苗的山羊最初对鹦鹉热衣原体产生特异性IgG1反应，而第二次和第三次接种诱导（如同衣原体流产的山羊）主要产生IgG1，但也产生IgG2。结果表明，通过分析特异性IgG1和IgG2反应，可将临床无症状的衣原体感染与显性疾病区分开来。对现场样本应用伯氏考克斯体特异性ELISA，在八只山羊中仅检测到IgG1，一只山羊中仅检测到IgG2，九只山羊中检测到IgG1和IgG2。与衣原体特异性反应相反，四只接种疫苗的山羊的考克斯体特异性抗体反应以IgG2占主导。
