School of Biosciences, Sir Martin Evans Building, Cardiff University, Wales, CF10 3AX, UK.
Function (Oxf). 2022 Dec 8;4(1):zqac061. doi: 10.1093/function/zqac061. eCollection 2023.
George Palade's pioneering electron microscopical studies of the pancreatic acinar cell revealed the intracellular secretory pathway from the rough endoplasmic reticulum at the base of the cell to the zymogen granules in the apical region. Palade also described for the first time the final stage of exocytotic enzyme secretion into the acinar lumen. The contemporary studies of the mechanism by which secretion is acutely controlled, and how the pancreas is destroyed in the disease acute pancreatitis, rely on monitoring molecular events in the various identified pancreatic cell types in the living pancreas. These studies have been carried out with the help of high-resolution fluorescence recordings, often in conjunction with patch clamp current measurements. In such studies we have gained much detailed information about the regulatory events in the exocrine pancreas in health as well as disease, and new therapeutic opportunities have been revealed.
乔治·帕拉德（George Palade）对胰腺腺泡细胞的开创性电子显微镜研究揭示了从细胞底部的粗面内质网到顶端区域的酶原颗粒的细胞内分泌途径。帕拉德还首次描述了酶原分泌到腺泡腔中的胞吐作用的最后阶段。目前对分泌如何被急性控制的机制的研究，以及胰腺在急性胰腺炎这种疾病中是如何被破坏的，都依赖于对活体胰腺中各种已鉴定的胰腺细胞类型中的分子事件进行监测。这些研究借助高分辨率荧光记录进行，通常与膜片钳电流测量相结合。在这些研究中，我们获得了有关健康和疾病中外分泌胰腺的调节事件的大量详细信息，并且揭示了新的治疗机会。
