Lee Sang-Jin, Kim Jeong-Min, Lee Eun Sun, Park Kwang-Yeol, Kim Hye Ryoun
Department of Neurology, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, Korea.
Department of Neurology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea.
J Clin Neurol. 2023 Jan;19(1):28-35. doi: 10.3988/jcn.2023.19.1.28.
We investigated whether circulating microRNAs (miRNAs) is associated with arterial stiffness in patients with acute ischemic stroke.
We recruited patients with acute ischemic stroke who were admitted to a university hospital stroke center and underwent carotid-femoral pulse wave velocity (cfPWV) measurement using SphygmoCor (AtCor Medical, Sydney, Australia) and brachial-ankle PWV using a volume-plethysmography device (VP-1000, Omron Colin, Komaki, Japan). Circulating miRNAs were measured in venous blood samples stored in EDTA. We selected five miRNAs (miR-17, miR-93, miR-450, miR-629, and let-7i) related to atherosclerosis based on a literature review. Pearson's correlation analysis was applied to the correlations between miRNAs and arterial stiffness parameters. Finally, multivariable linear regression analysis was performed to identify the independent factors for cfPWV.
This study included 70 patients (age=71.1±10.3 years [mean±SD], 29 females). The expression levels of miR-93 (=-0.27, =0.049) and let-7i (=-0.27, =0.039) were inversely correlated with cfPWV. Multivariable linear regression analysis including age, hypertension, and estimated glomerular filtration rate showed that let-7i was independently related with cfPWV (standardized coefficient=-0.262, =0.036). Correlation analysis indicated that let-7i was positively associated with visceral muscle Hounsfield units on computed tomography (=0.264, =0.043).
The expression level of let-7i was independently related to arterial stiffness in patients with cerebral infarction, suggesting that it plays a pathophysiological role in atherosclerosis.
我们研究了急性缺血性脑卒中患者循环微RNA（miRNA）是否与动脉僵硬度相关。
我们招募了入住大学医院卒中中心的急性缺血性脑卒中患者，使用SphygmoCor（澳大利亚悉尼AtCor Medical公司）测量颈股脉搏波速度（cfPWV），并使用体积描记装置（日本小牧欧姆龙科林公司VP - 1000）测量臂踝脉搏波速度。在储存于乙二胺四乙酸（EDTA）的静脉血样本中测量循环miRNA。基于文献综述，我们选择了5种与动脉粥样硬化相关的miRNA（miR - 17、miR - 93、miR - 450、miR - 629和let - 7i）。应用Pearson相关分析研究miRNA与动脉僵硬度参数之间的相关性。最后，进行多变量线性回归分析以确定cfPWV的独立影响因素。
本研究纳入70例患者（年龄 = 71.1±10.3岁[均值±标准差]，女性29例）。miR - 93（r = - 0.27，P = 0.049）和let - 7i（r = - 0.27，P = 0.039）的表达水平与cfPWV呈负相关。包括年龄、高血压和估算肾小球滤过率的多变量线性回归分析显示，let - 7i与cfPWV独立相关（标准化系数 = - 0.262，P = 0.036）。相关分析表明，let - 7i与计算机断层扫描上的内脏肌肉亨氏单位呈正相关（r = 0.264，P = 0.043）。
let - 7i的表达水平与脑梗死患者的动脉僵硬度独立相关，提示其在动脉粥样硬化中发挥病理生理作用。
