Pushkina N V, Tsybul'skiĭ I E, Lukash A I
Vopr Med Khim. 1987 Jul-Aug;33(4):52-5.
Rates of amidation of blood serum albumin and hemoglobin was studied in hyperglycemia developed during alloxan diabetes mellitus. Content of glucose in blood of the animals with diabetes exceeded 5.2-fold its level in blood of controls; amount of glycosylated hemoglobin was increased by 41.7%. Concentration of total, readily and hardly hydrolyzed amide groups was decreased in hemoglobin of the impaired animals by 13.1%, 17.6% and 10.4%, respectively, as compared with control group. The degree of blood serum albumin amidation was not altered. Hyperglycemia, accompanied by glycosylation of proteins, was the most probable cause of accelerated deamidation of hemoglobin. Participation of the protein deamidation in pathogenesis of diabetes mellitus is discussed.
研究了在四氧嘧啶糖尿病所致高血糖症中血清白蛋白和血红蛋白的酰胺化速率。糖尿病动物血液中的葡萄糖含量超过对照组血液中葡萄糖水平的5.2倍；糖化血红蛋白的量增加了41.7%。与对照组相比，受损动物血红蛋白中总酰胺基、易水解酰胺基和难水解酰胺基的浓度分别降低了13.1%、17.6%和10.4%。血清白蛋白的酰胺化程度未改变。伴有蛋白质糖基化的高血糖症是血红蛋白脱酰胺加速的最可能原因。文中讨论了蛋白质脱酰胺在糖尿病发病机制中的作用。
