生成携带 NKX2.5-EGFP 和 TBX5-Tdtomato 双荧光报告基因的人胚胎干细胞系 (SKLRMe004-A)。

Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters.

机构信息

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.

Department of Gynecology and Obstetrics, Affiliated Zhongda Hospital, Medical School, Southeast University, Nanjing, China.

出版信息

Stem Cell Res. 2023 Mar;67:103015. doi: 10.1016/j.scr.2023.103015. Epub 2023 Jan 2.

DOI:10.1016/j.scr.2023.103015

PMID:36608451

Abstract

TBX5 and NKX2-5 are two important transcription factors regulating cardiomyocytes commitment. In this study, we generated a dual fluorescent reporter cell line based on H9 human embryonic stem cells (hESCs) and called it H9-NKX2.5GFP/TBX5Td-T2A-18, which could be short for T2A-18. When T2A-18 was induced towards a cardiomyocyte fate, EGFP and Tdtomato fluorescences were observed, indicating the expression of NKX2.5 and TBX5, respectively. Meanwhile, T2A had normal karyotype and could maintain main characteristics of wildtype H9. Therefore, T2A-18 could be used as a tool to help us study the mechanism of cardiomyocytes specification from hESCs.

摘要

TBX5 和 NKX2-5 是两个调节心肌细胞分化的重要转录因子。在这项研究中，我们基于 H9 人胚胎干细胞（hESC）生成了一个双荧光报告细胞系，我们称之为 H9-NKX2.5GFP/TBX5Td-T2A-18，简称 T2A-18。当 T2A-18 被诱导向心肌细胞命运分化时，观察到 EGFP 和 Tdtomato 荧光，分别表示 NKX2.5 和 TBX5 的表达。同时，T2A 具有正常核型，能够维持野生型 H9 的主要特征。因此，T2A-18 可作为一种工具，帮助我们研究 hESC 向心肌细胞特化的机制。