常氧或低氧微环境中SAV1的表达谱与乳腺癌预后相关。
Profiles of Expression of SAV1 in Normoxia or Hypoxia Microenviroment are Associated with Breast Cancer Prognosis.
作者信息
de Amorim Ísis Salviano Soares, Dias Isabelle Xavier, Pinheiro Daphne, de Carvalho Simone Nunes, Nicolau-Neto Pedro, Rodrigues Juliana Alves, Siqueira Priscyanne Barreto, Oliveira Matheus da Silva, Panis Carolina, da Fonseca Adenilson de Souza, Mencalha Andre Luiz
机构信息
Laboratório de Biologia do Câncer, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Laboratório de Pesquisa em Células-Tronco, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
出版信息
Arch Med Res. 2023 Feb;54(2):79-85. doi: 10.1016/j.arcmed.2022.12.007. Epub 2023 Jan 4.
BACKGROUND
In breast cancer (BC), hypoxia is associated with poor prognosis. Protein Salvador homolog 1 (SAV1) acts as a tumor suppressor and is downregulated in the cancer cells. However, there is limited data on the expression profile of SAV1 and its importance in BC. It has not been studied to evaluate this phenomenon in a hypoxic microenvironment yet.
AIM
This study aimed to investigate SAV1 expression profiles under normoxia and hypoxia, and the potential of SAV1 in BC prognosis.
METHODS
Gene and protein expression analyses were performed using Real-Time quantitative PCR (RT-qPCR) and immunocytochemistry (ICC), respectively, and in silico analyses were performed using The Cancer Genome Atlas (TCGA). The survival curves were constructed using KMplotter.
RESULTS
SAV1 expression was lower in BC samples and tumor cell lines than in normal samples. The SAV1 mRNA levels were reduced in hypoxic estrogen receptor positive (ER) tumors, which were associated with a lower survival probability as compared to normoxic ER tumors. Furthermore, lower levels of SAV1 were found in advanced cancer stage samples, which are associated with worse survival curves and can be a risk factor for BC.
CONCLUSIONS
These data suggest a potential prognostic role of SAV1 in BC, with lower expressions associated with worse prognosis.
背景
在乳腺癌（BC）中，缺氧与预后不良相关。蛋白质萨尔瓦多同源物1（SAV1）作为一种肿瘤抑制因子，在癌细胞中表达下调。然而，关于SAV1的表达谱及其在乳腺癌中的重要性的数据有限。目前尚未在缺氧微环境中对这一现象进行研究。
目的
本研究旨在调查常氧和缺氧条件下SAV1的表达谱，以及SAV1在乳腺癌预后中的潜力。
方法
分别使用实时定量PCR（RT-qPCR）和免疫细胞化学（ICC）进行基因和蛋白质表达分析，并使用癌症基因组图谱（TCGA）进行生物信息学分析。使用KMplotter构建生存曲线。
结果
与正常样本相比，乳腺癌样本和肿瘤细胞系中SAV1的表达较低。缺氧雌激素受体阳性（ER）肿瘤中SAV1 mRNA水平降低，与常氧ER肿瘤相比，其生存概率较低。此外，在晚期癌症阶段样本中发现SAV1水平较低，这与较差的生存曲线相关，并且可能是乳腺癌的一个危险因素。
结论
这些数据表明SAV1在乳腺癌中具有潜在的预后作用，表达降低与预后不良相关。
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