Dreano M, Fouillet X, Brochot J, Vallet J M, Michel M L, Rungger D, Bromley P
Battelle Research Center, Geneva, Switzerland.
Virus Res. 1987 Jul;8(1):43-59. doi: 10.1016/0168-1702(87)90039-6.
The DNA fragment coding for the hepatitis B virus surface antigen (HBsAg) was placed under the control of a human 70 kDa heat-shock protein (hsp70) promotor sequence. This plasmid construct has been used in transfection experiments to establish a stable amnion cell line of human origin (Wish), expressing an HBsAg in a heat-regulated fashion. Post-translational modifications, such as assembly, glycosylation, secretion and production of both major and middle S proteins appear to function normally. In addition, production of HBsAg under various protocols of heat induction is described. After inoculation into nude mice, development of tumours has been observed at the site of injection. Tumour cells, dispersed by means of collagenase or trypsin treatment from excised tumours, and subsequently seeded into Petri dishes, were able to secrete the same quantities of HBsAg after heat induction as were cells of the original cell line.
编码乙肝病毒表面抗原（HBsAg）的DNA片段置于人70kDa热休克蛋白（hsp70）启动子序列的控制之下。该质粒构建体已用于转染实验，以建立一种稳定的人源羊膜细胞系（Wish），其以热调节方式表达HBsAg。翻译后修饰，如组装、糖基化、分泌以及主要和中等S蛋白的产生，似乎功能正常。此外，还描述了在各种热诱导方案下HBsAg的产生情况。接种到裸鼠体内后，在注射部位观察到肿瘤的形成。通过胶原酶或胰蛋白酶处理从切除的肿瘤中分散得到的肿瘤细胞，随后接种到培养皿中，在热诱导后能够分泌与原始细胞系细胞相同量的HBsAg。
