Department of Veterinary Biotechnology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad, 500030, India.
Arch Virol. 2023 Jan 7;168(2):36. doi: 10.1007/s00705-022-05674-6.
Viral pathogens are the primary cause of canine gastroenteritis. However, few structured comprehensive studies on the viral etiology of canine gastroenteritis have been conducted. In this study, 475 rectal swabs collected over three years (2018-2021) from clinical canine gastroenteritis cases were screened for the presence of six major enteric viruses - canine parvovirus 2 (CPV-2), canine distemper virus (CDV), canine adenovirus 2 (CAdV-2), canine coronavirus (CCoV), canine astrovirus (CaAstV), and canine rotavirus (CRV) - by real-time PCR. The most frequently detected virus was CPV-2, which was present in 64.8% of the samples (subtype 2a, 21.1%; 2b, 77.4%; 2c, 1.5%), followed by CDV (8%), CaAstV (7.2%), CCoV (5.9%), and CAdV-2 (4.6%). Two to four of these viruses in different combinations were found in 16.8% of the samples, and CRV was not detected. The complete genome sequences of Indian isolates of CDV, CCoV, and CaAstV were determined for the first time, and phylogenetic analysis was performed. This study highlights the need for routine prophylactic vaccination with the appropriate vaccines. Notably, 70.3% of animals vaccinated with DHPPiL were found to be positive for at least one virus. Hence, regular molecular analysis of the prevalent viruses is crucial for addressing vaccination failures.
病毒病原体是犬胃肠炎的主要病因。然而，针对犬胃肠炎的病毒病因学，目前仅有少数系统性综合研究。本研究对三年（2018-2021 年）间收集的 475 份来自临床犬胃肠炎病例的直肠拭子，采用实时 PCR 技术，针对六种主要的肠道病毒（犬细小病毒 2 型（CPV-2）、犬瘟热病毒（CDV）、犬腺病毒 2 型（CAdV-2）、犬冠状病毒（CCoV）、犬星状病毒（CaAstV）和犬轮状病毒（CRV））进行了检测。最常检测到的病毒是 CPV-2，在 64.8%的样本中存在（2a 亚型，21.1%；2b，77.4%；2c，1.5%），其次是 CDV（8%）、CaAstV（7.2%）、CCoV（5.9%）和 CAdV-2（4.6%）。在 16.8%的样本中发现了两种或两种以上的这些病毒的不同组合，而未检测到 CRV。本研究首次对 CDV、CCoV 和 CaAstV 的印度分离株进行了全基因组序列测定，并进行了系统发育分析。本研究强调了常规使用适当疫苗进行预防性接种的必要性。值得注意的是，70.3%接种 DHPPiL 的动物至少有一种病毒呈阳性。因此，定期对流行病毒进行分子分析对于解决疫苗接种失败至关重要。
