Rafeie Farjad, Abdoli Ramin, Hossein-Zadeh Navid Ghavi, Talebi Reza, Szmatoła Tomasz
Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.
Iran Silk Research Center, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Guilan, Iran.
Trop Anim Health Prod. 2023 Jan 7;55(1):34. doi: 10.1007/s11250-022-03448-5.
Gastrointestinal nematode (GINs) infections are one of the causative agents of health and economic issues in sheep production systems worldwide. Considerable genetic variations in resistance or susceptibility in different sheep breeds are documented, but published results are conflicting. Recent advances obtained by high-throughput technologies such as commercial SNP chips, whole-genome sequencing, or whole transcriptome profiling provide new insights into breeding for host resistance or nematode control at the genetic levels. This study aimed to identify potential biomarkers associated with the resistance to ovine GINs through a network analysis approach. Comprehensive gene and protein interaction networks were reconstructed for candidate genes involved in the most related immune pathways associated with resistance to ovine GINs using data mining from literature. Generally, 30 genes including CD53, CHIA, RELN, HRH1, EPS15, LRP8, ATP2B1, IL4, IL5, IL13, IL2RA, IL23R, TNFα, IFNγ, TBX21, SH3RF1, HERC2, PTPN1, BIN1, HERC5, C3AR1, NOS2, STAT5B, STAT4, CCL1, CCL8, VIL1, CXCR1, CXCR2, and CXCR4 located on chromosomes 1, 2, 3, 4, 5, 6, 11, 13, 19, and 20 have been found as containing effective regions with the most related pathways to nematode infections. The results obtained by network analysis showed two functional modules, belonging to the interleukins family (IL4, IL5, IL13, IL23R, and IL2RA) and chemokine receptors or ligands family (CXCR1, CXCR2, CXCR4, CCL1, and CCL8). Interleukins are a group of cytokines that are expressed by white blood cells with a major role in the immune system. Chemokines are also a family of chemoattractant cytokines which play a vital role in cell migration that influence the immune system by a process known as chemotaxis. The results provide useful information for the functional annotation of candidate genes related to parasite resistance and add new information towards a consensus on quantitative trait loci (QTLs) related to the incidence of nematode infections.
胃肠道线虫（GINs）感染是全球绵羊生产系统中健康和经济问题的致病因素之一。不同绵羊品种在抗性或易感性方面存在相当大的遗传变异，已有文献记载，但公布的结果相互矛盾。商业SNP芯片、全基因组测序或全转录组分析等高通量技术的最新进展，为在遗传水平上培育宿主抗性或控制线虫提供了新的见解。本研究旨在通过网络分析方法识别与绵羊抗GINs相关的潜在生物标志物。利用文献数据挖掘，为参与与绵羊抗GINs相关的最相关免疫途径的候选基因重建了综合基因和蛋白质相互作用网络。一般来说，位于1、2、3、4、5、6、11、13、19和20号染色体上的30个基因，包括CD53、CHIA、RELN、HRH1、EPS15、LRP8、ATP2B1、IL4、IL5、IL13、IL2RA、IL23R、TNFα、IFNγ、TBX21、SH3RF1、HERC2、PTPN1、BIN1、HERC5、C3AR1、NOS2、STAT5B、STAT4、CCL1、CCL8、VIL1、CXCR1、CXCR2和CXCR4，已被发现含有与线虫感染最相关途径的有效区域。网络分析结果显示了两个功能模块，分别属于白细胞介素家族（IL4、IL5、IL13、IL23R和IL2RA）和趋化因子受体或配体家族（CXCR1、CXCR2、CXCR4、CCL1和CCL8）。白细胞介素是一组由白细胞表达的细胞因子，在免疫系统中起主要作用。趋化因子也是一类趋化性细胞因子家族，在细胞迁移中起重要作用，通过一种称为趋化作用的过程影响免疫系统。这些结果为与寄生虫抗性相关的候选基因的功能注释提供了有用信息，并为与线虫感染发生率相关的数量性状位点（QTLs）的共识增加了新信息。
