Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223 Madrid, Spain.
Departamento de Ciencia de Materiales, ETSI Caminos, Canales y Puertos, Universidad Politécnica de Madrid, 28040 Madrid, Spain.
Molecules. 2022 Dec 27;28(1):226. doi: 10.3390/molecules28010226.
Single molecule interactions between biotin and streptavidin were characterized with functionalized DeepTip probes and used as a model system to develop a comprehensive methodology for the high-yield identification and analysis of single molecular events. The procedure comprises the covalent binding of the target molecule to a surface and of the sensing molecule to the DeepTip probe, so that the interaction between both chemical species can be characterized by obtaining force-displacement curves in an atomic force microscope. It is shown that molecular resolution is consistently attained with a percentage of successful events higher than 90% of the total number of recorded curves, and a very low level of unspecific interactions. The combination of both features is a clear indication of the robustness and versatility of the proposed methodology.
生物素和链霉亲和素之间的单分子相互作用采用功能化的 DeepTip 探针进行了表征，并将其作为模型系统，开发了一种全面的方法来高产量地识别和分析单分子事件。该方法包括将靶分子共价结合到表面上，以及将传感分子结合到 DeepTip 探针上，从而可以通过在原子力显微镜中获得力-位移曲线来表征两种化学物质之间的相互作用。结果表明，分子分辨率始终达到 90%以上的总记录曲线数的成功事件的百分比，并且特异性相互作用的水平非常低。这两个特征的结合清楚地表明了所提出的方法的稳健性和多功能性。
