通过选择离子监测法对哺乳动物脑和血液中的5-羟基哌啶酸和羟脯氨酸进行鉴定和定量分析。

Identification and quantification of 5-hydroxypipecolic acid and hydroxyproline in mammalian brain and blood by selected ion monitoring.

作者信息

Miyata T, Okano Y, Nagata-Tanoue J, Ijima-Miyamura S, Iwamura H, Takahama K, Hitoshi T

机构信息

Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.

出版信息

Anal Biochem. 1987 Jun;163(2):303-8. doi: 10.1016/0003-2697(87)90228-4.

DOI:10.1016/0003-2697(87)90228-4

PMID:3661981

Abstract

A method for the analysis of 5-hydroxypipecolic acid and 4-hydroxyproline in mammalian brain and blood is reported. The identification and quantification of the two hydroxyimino acids were accomplished with gas chromatography/mass spectrometry including a selected ion-monitoring technique following HPLC prepurification. The lower limit of detection for the method is 2 to 10 pmol. The amounts of 5-hydroxypipecolic acid and 4-hydroxyproline in blood were 20 to 30 pmol/ml and 3 to 6 nmol/ml, respectively. Their concentrations in the rabbit whole brain were determined to be 5 and 120 pmol/g, respectively.

摘要

报道了一种分析哺乳动物脑和血液中5-羟基哌啶酸和4-羟基脯氨酸的方法。这两种羟基亚氨基酸的鉴定和定量是通过气相色谱/质谱联用技术实现的，包括在高效液相色谱预纯化后采用选择离子监测技术。该方法的检测下限为2至10皮摩尔。血液中5-羟基哌啶酸和4-羟基脯氨酸的含量分别为20至30皮摩尔/毫升和3至6纳摩尔/毫升。它们在兔全脑中的浓度分别测定为5皮摩尔/克和120皮摩尔/克。