Löffler B M, Schmidt M, Kunze H
Max-Planck-Institut für Experimentelle Medizin, Göttingen, Federal Republic of Germany.
Anal Biochem. 1987 Jun;163(2):440-5. doi: 10.1016/0003-2697(87)90246-6.
A simple device has been developed for the disintegration of isolated cells in isotonic or hypertonic media. It is based on the extrusion of a cell suspension through a small orifice under controlled pressure. The diameter of this interchangeable orifice has to be adapted to the cell type to be disrupted. The conditions fulfill the requirements for an efficient disruption of the plasma membranes and for good preservation of the particularly fragile subcellular components, lysosomes and peroxisomes, of rat hepatocytes and rat Leydig cells.
已开发出一种简单装置，用于使等渗或高渗介质中的分离细胞解体。它基于在受控压力下将细胞悬液通过一个小孔挤出。这个可互换小孔的直径必须根据要破碎的细胞类型进行调整。这些条件满足高效破坏质膜以及良好保存大鼠肝细胞和大鼠睾丸间质细胞中特别脆弱的亚细胞成分（溶酶体和过氧化物酶体）的要求。
