Namikawa T, Nagai A, Takenaka O, Takenaka A
Laboratory of Animal Genetics, Faculty of Agriculture, Nagoya University, Japan.
Anim Genet. 1987;18(2):133-41. doi: 10.1111/j.1365-2052.1987.tb00752.x.
Two bovine haemoglobin beta chains, electrophoretically identical with the beta A chain of Herefords, were obtained from Ongole and Banteng, Bos javanicus, cattle. The amino acid residue differences of the two beta chains were compared by electrophoresis, cation-exchange and reverse-phase chromatography, amino acid analyses, and Edman degradation in comparison with beta A chain. The results showed that two beta chains differed from the beta A chain of the Hereford breed by the substitution of serine with threonine at the beta 43 position. No other difference was found between the two chains and beta A. This new beta chain type was termed beta A Zebu, which forms a possible evolutionarily transitional type between the beta A and the rare variant beta D Zambia found previously in African zebu cattle. The beta A Zebu differentiates from the previous beta B by at least four amino acid substitutions involving five codon-base changes.
从婆罗门牛（Bos javanicus）和爪哇野牛中获得了两条牛血红蛋白β链，它们在电泳上与赫里福德牛的βA链相同。通过电泳、阳离子交换和反相色谱法、氨基酸分析以及与βA链相比的埃德曼降解法，比较了这两条β链的氨基酸残基差异。结果表明，这两条β链与赫里福德品种的βA链的不同之处在于，在β43位置上苏氨酸取代了丝氨酸。这两条链与βA之间未发现其他差异。这种新的β链类型被称为βA瘤牛链，它可能是βA链与先前在非洲瘤牛中发现的罕见变体βD赞比亚链之间的一种进化过渡类型。βA瘤牛链与先前的βB链至少有四个氨基酸取代不同，涉及五个密码子碱基变化。
