Molecular Diagnostics, Texas A&M Veterinary Medical Diagnostic Laboratory, College Station, TX, USA.
Thermo Fisher Scientific, Austin, TX, USA.
J Vet Diagn Invest. 2023 Mar;35(2):178-181. doi: 10.1177/10406387221149407. Epub 2023 Jan 10.
Trichomonosis is a venereal disease of cattle caused by the protozoan infection in cattle herds can be economically costly for cattle producers; therefore, testing is important for detection of the agent. Given that bulls are considered to be subclinical carriers of , it is important to detect infection prior to movement and/or breeding season. We have described previously the development of an updated set of PCR primers and probes that offer increased sensitivity of detection in preputial washings collected in PBS by utilizing reverse-transcription real-time PCR (RT-rtPCR) that targets the 5.8S ribosomal RNA of the organism. Here, we report improvements in the updated RT-rtPCR reagents as well as the evaluation of testing of pooled preputial washings. We found that up to 5 preputial washings can be pooled, similar to routine testing practices (InPouch culture), without reducing the sensitivity of detection of
滴虫病是一种牛的性病，由原生动物感染引起。牛群中的感染可能对牛养殖者造成经济上的代价；因此，检测对于检测病原体非常重要。由于公牛被认为是 的亚临床携带者，因此在移动和/或繁殖季节之前检测 感染非常重要。我们之前已经描述了一组更新的 PCR 引物和探针的开发，该组引物和探针通过利用针对 生物 5.8S 核糖体 RNA 的逆转录实时 PCR（RT-rtPCR），在 PBS 中收集的包皮冲洗液中提高了 的检测灵敏度。在这里，我们报告了更新的 RT-rtPCR 试剂的改进以及对合并包皮冲洗液检测的评估。我们发现，多达 5 次包皮冲洗可以合并，类似于常规检测实践（InPouch 培养），而不会降低 的检测灵敏度。
