Department for Biomedical Science and Biofilms - Research Center for Biointerfaces, Faculty of Health and Society, Malmö University, 205 06Malmö, Sweden.
Institut Laue-Langevin (ILL), 38000Grenoble, France.
ACS Appl Mater Interfaces. 2023 Jan 25;15(3):3772-3780. doi: 10.1021/acsami.2c18956. Epub 2023 Jan 10.
Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, to gain access to sub-nanometer scale structural information on stable nanoparticle monolayers assembled on silicon crystals in a noncovalent manner using a Langmuir-Schaefer deposition. The structure of supported lipid bilayers formed on the nanoparticle arrays via vesicle fusion was investigated using a combination of grazing incidence X-ray and neutron scattering techniques complemented by fluorescence microscopy imaging. Ordered nanoparticle assemblies were shown to be suitable and stable substrates for the formation of curved and fluid lipid bilayers that retained lateral mobility, as shown by fluorescence recovery after photobleaching and quartz crystal microbalance measurements. Neutron reflectometry revealed the formation of high-coverage lipid bilayers around the spherical particles together with a flat lipid bilayer on the substrate below the nanoparticles. The presence of coexisting flat and curved supported lipid bilayers on the same substrate, combined with the sub-nanometer accuracy and isotopic sensitivity of grazing incidence neutron scattering, provides a promising novel approach to investigate curvature-dependent membrane phenomena on supported lipid bilayers.
纳米粒子支撑脂质双层（nanoSLB）阵列是脂质包覆的图案化纳米界面，为使用表面敏感技术研究弯曲的模型生物膜提供了一个平台。我们结合散射技术和直接成像，获得了关于使用 Langmuir-Schäfer 沉积以非共价方式在硅晶体上组装的稳定纳米粒子单层的亚纳米级结构信息。通过使用掠入射 X 射线和中子散射技术与荧光显微镜成像相结合，研究了通过囊泡融合在纳米粒子阵列上形成的支撑脂质双层的结构。有序的纳米粒子组装被证明是适合和稳定的弯曲和流体脂质双层形成的基底，如光漂白后荧光恢复和石英晶体微天平测量所示，保留了横向流动性。中子反射率测量揭示了在球形颗粒周围形成了高覆盖率的脂质双层，以及在纳米颗粒下方的基底上形成了平坦的脂质双层。在同一基底上存在共存的平坦和弯曲的支撑脂质双层，结合掠入射中子散射的亚纳米精度和同位素灵敏度，为研究支撑脂质双层上的曲率依赖性膜现象提供了一种很有前途的新方法。
