Università degli Studi Niccolò Cusano, Roma;
Università degli Studi Niccolò Cusano, Roma.
Epidemiol Prev. 2022 Sep-Dec;46(5-6):367-375. doi: 10.19191/EP22.5-6.A461.088.
the reopening of Universities in a COVID-19 pandemic context represented a potential source of virus transmission among students.
to measure the SARS-CoV-2 real circulation among university students attending the University of Rome 'Niccolò Cusano' by seroprevalence analysis.
retrospective study based on a point-of-care (POC) SARS-CoV-2 rapid qualitative serological screening performed on asymptomatic students attending the University.
between September 2020 to July 2021 at Niccolò Cusano University, 9,588 SARS-CoV-2 lateral-flow rapid qualitative antibody assays were performed on a total of 2,423 asymptomatic students. Among them, 389 individuals with compulsory attendance were tested every 10 days for a minimum observational period of 7 months.
the prevalence of SARS-CoV-2 IgM/IgG antibodies was estimated at more levels. It was calculated: 1. the number of positive cases detected among the total number of students tested during the screening period; 2. the cumulative seroprevalence over the time, and the seroprevalence distribution over the months; 3. the duration of seropositivity after SARS-CoV-2 infection in the known previous infected students repeatedly tested.
a total of 112 participants had a SARS-CoV-2 positive IgG and/or IgM antibodies test, 39 of them with a documented history of previous infection. In the remaining 73 cases, 24 were confirmed with an external quantitative serological analysis and identified as individuals with unknown previous SARS-CoV-2 exposure, 17 resulted false positive and 32 subjects were excluded. The total seroprevalence was 2.6% (95%CI 2.0%-3.3%) and among the 63 confirmed seropositive cases, 75% had detectable IgG antibodies, 3% had IgM antibodies, and 22% were positive for both IgM and IgG antibodies. In the 389 repeatedly-tested students, 36 students were positive to SARS-CoV-2 antibodies, 14 with unknown previous infection, and 22 with known previous infection. Among these, 50% retained immune memory up to 4 months post infection and 27% of cases retained seropositivity up to 7 months.
the data collected has been useful to measure a real epidemiological rate of the virus spread in a cohort of students in Italy as well as to obtain information on the antibodies seropositivity duration in individuals with previous infection.
在 COVID-19 大流行背景下，大学重新开放可能成为学生之间病毒传播的潜在来源。
通过血清流行率分析来衡量在罗马“尼古拉·库萨诺”大学就读的大学生中 SARS-CoV-2 的真实传播情况。
基于对无症状学生进行的即时护理（POC）SARS-CoV-2 快速定性血清学筛查的回顾性研究。
2020 年 9 月至 2021 年 7 月在尼古拉·库萨诺大学，对总共 2423 名无症状学生进行了 9588 次 SARS-CoV-2 侧向流动快速定性抗体检测。其中，对 389 名必须出勤的个体进行了每 10 天一次的测试，观察期至少为 7 个月。
在多个层面上估计 SARS-CoV-2 IgM/IgG 抗体的流行率。它是通过以下方式计算的：1. 在筛查期间检测的所有学生中检测到的阳性病例数；2. 随时间推移的累积血清阳性率，以及随月份分布的血清阳性率；3. 在已知以前感染过的学生中反复检测到 SARS-CoV-2 感染后，血清阳性持续时间。
共有 112 名参与者的 SARS-CoV-2 阳性 IgG 和/或 IgM 抗体检测呈阳性，其中 39 名有记录的既往感染史。在其余 73 例中，24 例通过外部定量血清学分析确认，并确定为以前 SARS-CoV-2 暴露未知的个体，17 例为假阳性，32 例被排除。总血清阳性率为 2.6%（95%CI 2.0%-3.3%），在 63 例确诊的血清阳性病例中，75%有可检测的 IgG 抗体，3%有 IgM 抗体，22%同时有 IgM 和 IgG 抗体阳性。在 389 名反复检测的学生中，有 36 名 SARS-CoV-2 抗体检测呈阳性，其中 14 名以前的感染情况未知，22 名以前的感染情况已知。在这些学生中，50%的人在感染后 4 个月内保留了免疫记忆，27%的人在感染后 7 个月内保持了血清阳性。
收集的数据有助于衡量意大利学生群体中病毒传播的真实流行率，并获得以前感染个体抗体血清阳性持续时间的信息。
