通过简单的咪唑衍生发光体快速荧光检测胆红素及其在加标生物体液中的实用适用性。
Expeditious fluorimetric detection of bilirubin by simple imidazole derived luminophore and it's pragmatic applicability in spiked biological fluids.
机构信息
Organic and Polymer Synthesis Laboratory, Department of Chemistry, National Institute of Technology, Tiruchirappalli, 620 015, India.
Organic and Polymer Synthesis Laboratory, Department of Chemistry, National Institute of Technology, Tiruchirappalli, 620 015, India.
出版信息
Anal Chim Acta. 2023 Jan 25;1239:340678. doi: 10.1016/j.aca.2022.340678. Epub 2022 Nov 26.
Bilirubin is an indispensable biomarker for liver diseases. Utilizing organic molecules as sensor platform for effective detection of bilirubin are little. In addition, the reported fluorophores required longer incubation time for detection. Hence, herein we have attempted to design an imidazole derivative 4-(3H-imidazo[4,5-b]pyridin-2-yl)-N,N-diphenylaniline (IMI) from triphenylamine and pyridine units which could detect bilirubin swiftly without any incubation period. IMI manifested an instant quenching of emission in presence of bilirubin with limit of detection (LOD) 11.74 × 10 mol L. The mechanistic aspect of detection involves coexistence of both static and dynamic quenching which was suitably justified. Finally, the pragmatic application of IMI was performed in bio-fluids.
胆红素是肝脏疾病不可缺少的生物标志物。利用有机分子作为传感器平台来有效检测胆红素的方法很少。此外，已报道的荧光团在检测时需要更长的孵育时间。因此，本文尝试设计了一种基于三苯胺和吡啶单元的咪唑衍生物 4-(3H-咪唑并[4,5-b]吡啶-2-基)-N,N-二苯基苯胺（IMI），它可以在没有任何孵育期的情况下快速检测胆红素。IMI 在存在胆红素时表现出即时的发射猝灭，检测限（LOD）为 11.74×10-6mol L-1。检测的机理涉及静态和动态猝灭的共存，这得到了合理的解释。最后，在生物流体中进行了 IMI 的实际应用。
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