Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India.
Biotechnology Division, Defence Research Development & Establishment, Jhansi Road, Gwalior 474002, Madhya Pradesh, India.
Biosens Bioelectron. 2017 May 15;91:82-88. doi: 10.1016/j.bios.2016.12.026. Epub 2016 Dec 11.
Bilirubin, a key biomarker for the jaundice and its clinical diagnosis needs a better analytical tool. A novel and simple fluorescent platform based on (2,2'-((1E,1'E)-((6-bromopyridine-2,3-diyl) bis(azanylylidene)) bis(methanylylidene diphenol) (BAMD) was designed. BAMD showed a remarkable fluorescent intensity with a very good quantum yield of 0.85 and lifetime of 870ps. Hence, it was applied for the determination of bilirubin using both colorimetric and fluorimetric techniques in physiological and basic pH. Under optimized experimental conditions, the probe detects bilirubin selectively in the presence of other interfering biomolecules and metal ions. The linear range of detection is 1pM-500µM at pH=7.4 and LOD is 2.8 and 3.3 pM at pH=7.4 and 9.0, respectively, which were reported so far. The probe detects the bilirubin through FRET mechanism. The practical application of the probe was successfully tested in the human blood and urine samples. Based on all above advantages, this simple idea can be applied to design a simple clinical diagnostic tool for jaundice.
胆红素是黄疸的一个关键生物标志物，其临床诊断需要更好的分析工具。本研究设计了一种基于（2,2'-((1E,1'E)-((6-溴吡啶-2,3-二基)双(氮杂亚基))双(亚甲基)二苯酚) (BAMD)的新型简单荧光平台。BAMD 表现出显著的荧光强度，具有非常好的量子产率为 0.85 和寿命为 870ps。因此，它被应用于在生理和基础 pH 下使用比色法和荧光法测定胆红素。在优化的实验条件下，探针在存在其他干扰生物分子和金属离子的情况下选择性地检测胆红素。在 pH=7.4 时，检测范围为 1pM-500µM，LOD 分别为 2.8 和 3.3 pM 在 pH=7.4 和 9.0 时，这是迄今为止报道的最低检测限。探针通过 FRET 机制检测胆红素。该探针在人血液和尿液样本中的实际应用成功进行了测试。基于所有这些优点，这个简单的想法可以应用于设计用于黄疸的简单临床诊断工具。
