Raza A A, Mushtaq R, Khwaja S, Akram A, Karim A, Akhter A
Federal Urdu University of Arts Science and Technology, Department of Biochemistry, Karachi, Pakistan.
Federal Urdu University of Arts Science and Technology, Department of Zoology, Karachi, Pakistan.
Braz J Biol. 2023 Jan 6;84:e266940. doi: 10.1590/1519-6984.266940. eCollection 2023.
This is a case control and cohort study, conducted at Department of Biochemistry in FUAST, compressing 24 number of albino Wistar rats. Illicium verum (star anise) natural spice and green vegetable broccoli are used for chemo-preventive herbal treatment in relation with vitamin C antioxidant activity. Hepatotoxicity was induced in liver of model rats by giving interperitoneally single injection of 200 mg/kg bod weight (B.W) of N-nitrosodiethylamine and orally giving 1 mg/kg BW phenobarbital for 14 days during disease prorogation period. The vitamin C analysis from Illicium verum (star anise), green broccoli, orange juice, orange peel and orange pulp was done by iodometric quantitative and qualitative titration method. Rats were given compound according to their bodyweight as 1 mg/kg B.W. After 14 days (Disease Promotion and Propagation Period) and 28 days (Disease cure period) of treatment behavioral activity was monitored by locomotors activity in open field experiment, stimulatory activity in home cage and anxiolytic effects observed in light and dark apparatus and also in T maze. Behavioral activity were significantly increased in Illicium verum and green broccoli treated rats. Hematological study including hemoglobin (Hb), white blood cells (WBC), red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW) and Platelets was done by abbot laboratory analyzer cell. The value of Hb and RBC was also significantly increased in star anise and broccoli treated rats, showed proper function of RBC in microcirculation. WBC was also in normal range revealed that no disease regards to bone marrow and leukemia's. The biochemical activity of rats including urea, creatinine, total protein, albumin, globulin, A/G ratio, amylase and calcium in blood serum were analyzed while, liver health or performance was also determined by LFT (liver function test). Behavioral and laboratorial variables were analyzed by using SPSS v. 20 and p-value < 0.05 was considered statistically significant. The study report noticeable significant effect of Illicium verum and broccoli on hepatotoxicity of experimental model. Recent experimental study statistical outcomes show reciprocal relationship between the consumption of vegetable diet and natural spice with the risk of Hepatotoxicity. Thus, the aim of the study is to enhance the function of star anise or illicium verum natural spice and green vegetable broccoli as an anticancer or chemoprophylaxis agent.
这是一项在费萨拉巴德农业大学（FUAST）生物化学系进行的病例对照和队列研究，涉及24只白化Wistar大鼠。将八角茴香（大茴香）这种天然香料和绿色蔬菜西兰花用于与维生素C抗氧化活性相关的化学预防草药治疗。在疾病传播期，通过腹腔注射200毫克/千克体重的N-亚硝基二乙胺，并口服1毫克/千克体重的苯巴比妥，连续14天，诱导模型大鼠肝脏产生肝毒性。采用碘量法定量和定性滴定法对八角茴香（大茴香）、绿色西兰花、橙汁、橙皮和橙肉中的维生素C进行分析。根据大鼠体重，按1毫克/千克体重给它们喂食化合物。在治疗14天（疾病促进和传播期）和28天（疾病治愈期）后，通过旷场实验中的运动活动、家笼中的刺激活动以及在明暗箱和T迷宫中观察到的抗焦虑作用来监测行为活动。八角茴香和绿色西兰花处理的大鼠行为活动显著增加。通过雅培实验室分析仪细胞进行血液学研究，包括血红蛋白（Hb）、白细胞（WBC）、红细胞（RBC）、平均红细胞体积（MCV）、平均红细胞血红蛋白（MCH）、平均红细胞血红蛋白浓度（MCHC）、红细胞分布宽度（RDW）和血小板。八角茴香和西兰花处理的大鼠中Hb和RBC的值也显著增加，表明红细胞在微循环中功能正常。白细胞也在正常范围内，表明没有与骨髓和白血病相关的疾病。分析大鼠血清中的生化活性，包括尿素、肌酐、总蛋白、白蛋白、球蛋白、A/G比值、淀粉酶和钙，同时通过肝功能测试（LFT）确定肝脏健康状况或性能。使用SPSS v. 20对行为和实验室变量进行分析，p值<0.05被认为具有统计学意义。研究报告显示八角茴香和西兰花对实验模型的肝毒性有显著影响。最近的实验研究统计结果表明，蔬菜饮食和天然香料的摄入与肝毒性风险之间存在相互关系。因此，该研究的目的是增强八角茴香或大茴香这种天然香料以及绿色蔬菜西兰花作为抗癌或化学预防剂 的功能。
