来自长期糖尿病大鼠心脏的分离心肌细胞中的三酰甘油脂肪酶活性。

Triacylglycerol lipase activities in isolated myocardial cells from chronically diabetic rat hearts.

作者信息

Severson D L, Larsen T S, Ramírez I

机构信息

Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Calgary, Canada.

出版信息

Basic Res Cardiol. 1987;82 Suppl 1:37-43. doi: 10.1007/978-3-662-08390-1_5.

DOI:10.1007/978-3-662-08390-1_5

PMID:3663009

Abstract

Diabetes was induced by the administration of streptozotocin (55 mg/kg or 70 mg/kg) to rats. After 21-25 days, myocardial cells (myocytes) were isolated from control and diabetic rat hearts. Rates of endogenous lipolysis, measured as the output of glycerol, were elevated in the chronically diabetic myocytes. Lipoprotein lipase activity was reduced in homogenates of diabetic myocytes. Neutral triacylglycerol lipase activity was increased in myocytes from rats made diabetic with the lower dose of streptozotocin, but not in myocytes from diabetic rats given the higher dose. Diabetes had no effect on acid lysosomal lipase activity.

摘要

通过给大鼠注射链脲佐菌素（55毫克/千克或70毫克/千克）诱导糖尿病。21至25天后，从对照大鼠和糖尿病大鼠心脏中分离出心肌细胞（心肌细胞）。以内源性甘油释放量衡量的脂肪分解率在慢性糖尿病心肌细胞中升高。糖尿病心肌细胞匀浆中的脂蛋白脂肪酶活性降低。低剂量链脲佐菌素诱导的糖尿病大鼠心肌细胞中的中性三酰甘油脂肪酶活性增加，但高剂量链脲佐菌素诱导的糖尿病大鼠心肌细胞中的该酶活性未增加。糖尿病对酸性溶酶体脂肪酶活性没有影响。