Department of Otolaryngology, Head and Neck Surgery, Yantai Yuhuangding Hospital, Qingdao University, Yantai, China; Shandong Provincial Clinical Research Center for Otorhinolaryngologic Diseases, Yantai Yuhuangding Hospital, Qingdao University, Yantai, China.
Department of Otolaryngology, Head and Neck Surgery, Yantai Yuhuangding Hospital, Qingdao University, Yantai, China; Shandong Provincial Clinical Research Center for Otorhinolaryngologic Diseases, Yantai Yuhuangding Hospital, Qingdao University, Yantai, China; Binzhou medical university, Department of clinical medicine, Yantai, China.
Arch Oral Biol. 2023 Mar;147:105615. doi: 10.1016/j.archoralbio.2023.105615. Epub 2023 Jan 7.
Head and neck squamous cell carcinoma (HNSCC), is one of the malignant tumors with high recurrence and metastasis. The family with sequence similarity (FAM) of non-coding RNAs promoted tumorigenesis and metastasis. But so far, long non-coding RNA (lncRNA) FAM239A's function in HNSCC regulation remains unclear. This study aimed to explore the lncRNA FAM239A function and regulation mechanism in HNSCC cell proliferation and migration.
The expression level of lncRNA FAM239A and tyrosine phosphatase Src homology 2 domain-containing phosphatase 2 (SHP2) in HNSCC tumor tissue was tested by quantitative polymerase chain reaction. The cell proliferation and migration were tested by cell counting kit 8, kinetic live cell assay, and wound healing assay. The differential expression of SHP2 and immune infiltration in HNSCC were analyzed in the tumor immune estimation response and human protein atlas databases. And the survival analysis of SHP2 in HNSCC was analyzed in the gene expression profiling interactive analysis 2 databases. The SHP2 expression was tested by western blotting when lncRNA FAM239A overexpression and knockdown.
LncRNA FAM239A and SHP2 were ectopically expressed in HNSCC tumor tissue. Cell proliferation and wound healing assays showed that lncRNA FAM239A promoted tumor cell proliferation and migration. SHP2 was overexpressed in HNSCC tumor tissue by database analyses, and the higher SHP2 expression caused poorer overall survival and disease-free survival in HNSCC patients. SHP2 expression was positively regulated by lncRNA FAM239A.
LncRNA FAM239A promoted HNSCC cell proliferation and migration through upregulating SHP2 expression, which potentially provided new regulators for HNSCC.
头颈部鳞状细胞癌（HNSCC）是一种复发和转移率较高的恶性肿瘤。非编码 RNA 家族相似性（FAM）促进了肿瘤的发生和转移。但迄今为止，长链非编码 RNA（lncRNA） FAM239A 在 HNSCC 调控中的作用尚不清楚。本研究旨在探讨 lncRNA FAM239A 在 HNSCC 细胞增殖和迁移中的功能和调控机制。
通过定量聚合酶链反应检测 HNSCC 肿瘤组织中 lncRNA FAM239A 和酪氨酸磷酸酶 Src 同源性 2 结构域含磷酶 2（SHP2）的表达水平。通过细胞计数试剂盒 8、动力学活细胞测定和划痕愈合试验检测细胞增殖和迁移。在肿瘤免疫评估反应和人类蛋白质图谱数据库中分析 HNSCC 中 SHP2 的差异表达和免疫浸润。并在基因表达谱交互式分析 2 数据库中分析 SHP2 在 HNSCC 中的生存分析。当 lncRNA FAM239A 过表达和敲低时，通过 Western blot 检测 SHP2 的表达。
lncRNA FAM239A 和 SHP2 在 HNSCC 肿瘤组织中异常表达。细胞增殖和划痕愈合试验表明，lncRNA FAM239A 促进肿瘤细胞增殖和迁移。数据库分析显示 SHP2 在 HNSCC 肿瘤组织中过表达，SHP2 表达较高的 HNSCC 患者总生存率和无病生存率较差。SHP2 的表达受 lncRNA FAM239A 的正向调节。
lncRNA FAM239A 通过上调 SHP2 的表达促进 HNSCC 细胞的增殖和迁移，这可能为 HNSCC 提供了新的调控因子。
