绵羊肌肉中乙酸盐与乙酰辅酶A之间底物循环速率的体内测量。乙酸盐输注的影响。
Measurement of the rate of substrate cycling between acetate and acetyl-CoA in sheep muscle in vivo. Effects of infusion of acetate.
作者信息
Crabtree B, Marr S A, Anderson S E, MacRae J C
机构信息
Rowett Research Institute, Bucksburn, Aberdeen, Scotland, U.K.
出版信息
Biochem J. 1987 May 1;243(3):821-7. doi: 10.1042/bj2430821.
Abstract
- The rate of substrate cycling between acetate and acetyl-CoA in sheep hindlimb muscle was measured from the decrease of [14C]acetate specific radioactivity across the tissue in vivo. 2. The rate of cycling was 0.005-0.015 mumol/min per g and, in two of the three sheep investigated, was approximately doubled as a result of infusing 2 mol acetate/day intravenously. 3. The results indicate that this cycle contributes only approx. 0.5% of the total heat produced by the animal and would probably make only a relatively small contribution to any heat increment of feeding associated with diets producing large amounts of acetate.
摘要
- 通过测量体内绵羊后肢肌肉中[14C]乙酸盐比放射性在组织中的降低情况，来测定乙酸盐与乙酰辅酶A之间的底物循环速率。2. 循环速率为每克0.005 - 0.015微摩尔/分钟，在三只被研究绵羊中的两只中，由于每天静脉注射2摩尔乙酸盐，循环速率大约增加了一倍。3. 结果表明，这个循环仅约占动物产生的总热量的0.5%，并且对于与产生大量乙酸盐的日粮相关的任何采食热增量可能仅做出相对较小的贡献。
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