Crabtree B, Souter M J, Anderson S E
Rowett Research Institute, Bucksburn, Aberdeen, U.K.
Biochem J. 1989 Feb 1;257(3):673-8. doi: 10.1042/bj2570673.
By using [1-14C]butyrate, the fluxes of butyrate to acetate and fatty acids were measured in rat hepatocytes. Both fluxes were inhibited to a similar extent by (-)-hydroxycitrate, with no significant effect on butyrate uptake. These results indicate that acetate formation takes place in the cytoplasm, presumably via ATP-stimulated acetyl-CoA hydrolase. Since acetate formation occurred despite a net uptake of acetate, the results are also consistent with the operation of a substrate cycle between acetate and acetyl-CoA, recently proposed by other workers, and suggest that this cycle is cytoplasmic.
通过使用[1-14C]丁酸，在大鼠肝细胞中测量了丁酸向乙酸和脂肪酸的通量。（-）-羟基柠檬酸对这两种通量的抑制程度相似，对丁酸摄取无显著影响。这些结果表明，乙酸的形成发生在细胞质中，推测是通过ATP刺激的乙酰辅酶A水解酶。由于尽管有乙酸的净摄取仍发生了乙酸的形成，这些结果也与其他研究人员最近提出的乙酸和乙酰辅酶A之间底物循环的运作一致，并表明该循环是在细胞质中的。
