大鼠脑内磷脂甲基化途径产生的磷脂酰胆碱的分子组成（体内研究）
Molecular composition of the phosphatidylcholines produced by the phospholipid methylation pathway in rat brain in vivo.
作者信息
Lakher M B, Wurtman R J
机构信息
Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
Biochem J. 1987 Jun 1;244(2):325-30. doi: 10.1042/bj2440325.
We examined the formation in vivo of molecular subspecies of brain phosphatidylcholine (PC) via the phospholipid-methylation pathway. [3H]Methionine was infused into a lateral cerebral ventricle, and 3H-labelled PC was isolated from brains of rats 0.1-18 h after the infusions. Three major subspecies of this PC, differing in their fatty acid compositions, were separated on silver-impregnated t.l.c. plates, and the proportions of radioactivities in these three PC fractions were determined. The results indicate that newly-formed PC synthesized by methylation of phosphatidylethanolamine at 0.1 h after [3H]methionine contains a significantly higher proportion of polyunsaturated subspecies (i.e. those with six or four double bonds) than does PC obtained later times after injection of [3H]methionine. This change in the composition of 3H-labelled brain PC occurs gradually and is not due to an influx of radioactive PC from the periphery. Our data suggest that polyunsaturated PC (hexaenes and tetraenes) produced in the brain by methylation of phosphatidylethanolamine turns over faster than does that containing more-saturated fatty acids.
我们通过磷脂甲基化途径研究了脑磷脂酰胆碱（PC）分子亚类在体内的形成。将[³H]甲硫氨酸注入大脑侧脑室，并在注入后0.1 - 18小时从大鼠脑中分离出³H标记的PC。这种PC的三个主要亚类，其脂肪酸组成不同，在浸银薄层层析板上被分离，并测定了这三个PC组分中的放射性比例。结果表明，在[³H]甲硫氨酸注入后0.1小时通过磷脂酰乙醇胺甲基化合成的新形成的PC，与在注入[³H]甲硫氨酸后较晚时间获得的PC相比，含有显著更高比例的多不饱和亚类（即具有六个或四个双键的那些）。³H标记的脑PC组成的这种变化是逐渐发生的，并且不是由于放射性PC从外周流入所致。我们的数据表明，通过磷脂酰乙醇胺甲基化在脑中产生的多不饱和PC（六烯和四烯）的周转速度比含有更多饱和脂肪酸的PC更快。
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