Garfinkel L, Garfinkel D, Matsiras P, Matschinsky B
Department of Computer Science, University of Pennsylvania, Philadelphia 19104.
Biochem J. 1987 Jun 1;244(2):351-7. doi: 10.1042/bj2440351.
We have constructed a relational data base containing the kinetic properties of the isoenzymes of hexokinase using the Knowledgeman data-base program on an IBM PC microcomputer. The natural subunit of this data base is the refereed publication, 165 of which were included. Reported values for the Mr (approx. 97,000) are in good agreement, but this agreement becomes progressively worse as one examines the Km values for glucose and ATP and the Ki for glucose 6-phosphate, where the reported values are spread over three orders of magnitude. Some quantities are very thinly or unreliably determined. Experimental conditions, especially free Mg2+ concentration, are rarely close to physiological. Reasons for the spread or uncertainty of numbers, and the distinctions that can be made between isoenzymes despite this spread, are discussed.
我们使用IBM个人计算机上的Knowledgeman数据库程序构建了一个包含己糖激酶同工酶动力学特性的关系数据库。该数据库的自然单元是经过评审的出版物，共纳入了165篇。报道的相对分子质量（约97,000）值吻合良好，但当查看葡萄糖和ATP的米氏常数（Km）以及6-磷酸葡萄糖的抑制常数（Ki）时，这种吻合度逐渐变差，报道值分布在三个数量级范围内。一些量的测定非常稀疏或不可靠。实验条件，尤其是游离镁离子浓度，很少接近生理状态。文中讨论了数据分散或不确定的原因，以及尽管存在这种分散情况但仍可在同工酶之间做出区分的依据。
