Garfinkel D, Garfinkel L, Meglasson M D, Matschinsky F M
Am J Physiol. 1984 Sep;247(3 Pt 2):R527-36. doi: 10.1152/ajpregu.1984.247.3.R527.
Previous work from this laboratory indicates that glucokinase serves as the glucose sensor of pancreatic islets. Here we show by nonlinear computer optimization that the kinetic properties of glucokinase (together with hexokinase, known to be present in islets) account for the observed glycolytic rates in islets as a function of glucose level. Alternative enzymes that have been suggested to perform the same function as glucokinase, N-acetyl-D-glucosamine kinase and glucose-6-phosphatase, are shown to have incompatible properties, including a poor fit, different curve shapes, and unreasonable parameter values resulting from optimization. Their activities in islets are shown to be too low to account for observed glucose usage rates. This work endorses our previous proposal that glucokinase acts as the glucose sensor in pancreatic islet cells.
该实验室之前的研究表明，葡萄糖激酶是胰岛的葡萄糖传感器。在此我们通过非线性计算机优化表明，葡萄糖激酶（以及已知存在于胰岛中的己糖激酶）的动力学特性可解释观察到的胰岛糖酵解速率随葡萄糖水平的变化。有人提出N - 乙酰 - D - 葡萄糖胺激酶和葡萄糖 - 6 - 磷酸酶可执行与葡萄糖激酶相同的功能，但研究表明它们具有不相容的特性，包括拟合不佳、曲线形状不同以及优化后产生的不合理参数值。它们在胰岛中的活性过低，无法解释观察到的葡萄糖利用率。这项研究支持了我们之前提出的关于葡萄糖激酶在胰岛细胞中作为葡萄糖传感器的提议。
