3rd Department of Surgery, Attikon University Hospital, Athens, Greece.
Institute of Pathology, National and Kapodistrian University of Athens, Athens, Greece.
Pediatr Surg Int. 2023 Jan 11;39(1):80. doi: 10.1007/s00383-022-05341-6.
Nowadays, the standard therapy for patients with short bowel syndrome is parenteral nutrition (PN). Various growth factors have been tested to achieve weaning from prolonged PN administration. We evaluated the effect of hepatocyte growth factor (HGF) on structural intestinal adaptation and cell proliferation in a rat model of SBS.
Thirty Sprague-Dawley rats were divided into three groups; group A rats (sham) underwent bowel transection, group B rats underwent a 75% bowel resection, and group C rats underwent the same procedure but were treated postoperatively with HGF. Histopathologic parameters of intestinal adaptation were determined, while microarray and rt-PCR analyses of ileal RNA were also performed.
Treatment with HGF resulted in significant increase in body weight, while the jejunal and ileal villus height and crypt depth were increased in HGF rats (36%, p < 0.05 and 27%, p < 0.05 respectively). Enterocyte proliferation was also significantly increased in HGF rats (21% p < 0.05). Microarray and quantitative rt-PCR analyses showed that the genes hgfac, rac 1, cdc42, and akt 1 were more than twofold up-regulated after HGF treatment.
HGF emerges as a growth factor that enhances intestinal adaptation. The future use of HGF may potentially reduce the requirement for PN in SBS patients.
目前，短肠综合征患者的标准治疗方法是肠外营养（PN）。已经测试了各种生长因子以实现从长期 PN 给药中逐渐脱离。我们评估了肝细胞生长因子（HGF）在 SBS 大鼠模型中对结构肠道适应和细胞增殖的影响。
将 30 只 Sprague-Dawley 大鼠分为三组；A 组大鼠（假手术）行肠切断术，B 组大鼠行 75%肠切除术，C 组大鼠行相同手术，但术后给予 HGF 治疗。确定肠道适应的组织病理学参数，同时还进行了回肠 RNA 的微阵列和 rt-PCR 分析。
HGF 治疗导致体重显著增加，而 HGF 大鼠的空肠和回肠绒毛高度和隐窝深度增加（分别增加 36%，p<0.05 和 27%，p<0.05）。HGF 大鼠的肠细胞增殖也显著增加（21%，p<0.05）。微阵列和定量 rt-PCR 分析表明，HGF 治疗后 hgfac、rac1、cdc42 和 akt1 基因的表达上调了两倍以上。
HGF 作为一种生长因子，可增强肠道适应。未来 HGF 的应用可能会降低 SBS 患者对 PN 的需求。
