Division of Physiome, Department of Physiology, Hyogo Medical University, 1-1 Mukogawa-cho, Nishinomiya, 663-8501, Japan.
Department of Statistical Modeling, The Institute of Statistical Mathematics, 10-3, Midori-cho, Tachikawa, 190-0014, Japan.
Sci Rep. 2023 Jan 11;13(1):586. doi: 10.1038/s41598-023-27893-w.
To examine whether and how the inspiratory neuronal network in the preBötzinger complex (preBötC) develops during the early postnatal period, we quantified the composition of the population of inspiratory neurons between postnatal day 1 (p1) and p10 by applying calcium imaging to medullary transverse slices in double-transgenic mice expressing fluorescent marker proteins. We found that putative excitatory and glycinergic neurons formed a majority of the population of inspiratory neurons, and the composition rates of these two inspiratory neurons inverted at p5-6. We also found that the activity patterns of these two types of inspiratory neurons became significantly well-synchronized with the inspiratory rhythmic bursting pattern in the preBötC within the first postnatal week. GABAergic and GABA-glycine cotransmitting inspiratory neurons formed only a small population just after birth, which almost disappeared until p10. In conclusion, the inspiratory neuronal network in the preBötC matures at the level of both neuronal population and neuronal activities during early postnatal development.
为了研究吸气神经元网络在 preBötzinger 复合体（preBötC）中的发育情况及其机制，我们通过对双转基因小鼠的延髓横切片进行钙成像，在出生后第 1 天（p1）至第 10 天（p10）之间，定量分析了吸气神经元的群体组成。结果发现，在 p5-6 时，假定的兴奋性神经元和甘氨酸能神经元构成了吸气神经元群体的主要部分，这两种吸气神经元的组成率发生了反转。我们还发现，在出生后的第一周内，这两种类型的吸气神经元的活动模式与 preBötC 中的吸气节律性爆发模式变得明显同步。GABA 能和 GABA-甘氨酸共传递的吸气神经元在出生后不久仅形成一小部分，直到 p10 几乎消失。总之，在出生后的早期发育过程中，preBötC 中的吸气神经元网络在神经元群体和神经元活动水平上都趋于成熟。
