利用反相高压液相色谱法分离谷胱甘肽转移酶亚基。
The separation of glutathione transferase subunits by using reverse-phase high-pressure liquid chromatography.
作者信息
Ostlund Farrants A K, Meyer D J, Coles B, Southan C, Aitken A, Johnson P J, Ketterer B
机构信息
Department of Biochemistry, Middlesex Hospital Medical School, London, U.K.
出版信息
Biochem J. 1987 Jul 15;245(2):423-8. doi: 10.1042/bj2450423.
Abstract
A simple method is described for the separation and quantification of the subunits of GSH transferases present in rat tissue extracts. This method, involving GSH-agarose affinity chromatography followed by reverse-phase h.p.l.c., is rapid and sufficiently sensitive to measure 5 micrograms of each subunit in a mixture. Examples are given of its application to extracts of rat kidney, adrenal, testicular interstitial cells and seminiferous tubules. The analysis of seminiferous tubules indicates that the technique may be of value for the identification of novel subunits. Preliminary separations of subunits from human GSH transferases are also described.
摘要
本文描述了一种简单的方法，用于分离和定量大鼠组织提取物中谷胱甘肽转移酶（GSH transferases）的亚基。该方法包括谷胱甘肽琼脂糖亲和色谱，随后进行反相高效液相色谱，快速且灵敏度足以测量混合物中每个亚基5微克的含量。文中给出了该方法应用于大鼠肾脏、肾上腺、睾丸间质细胞和生精小管提取物的示例。生精小管的分析表明，该技术可能对鉴定新亚基有价值。文中还描述了从人谷胱甘肽转移酶中初步分离亚基的情况。
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本文引用的文献
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Evidence that the Ya and Yc subunits of glutathione transferase B (ligandin) are the products of separate genes.谷胱甘肽转移酶B（配体结合蛋白）的Ya和Yc亚基是不同基因产物的证据。
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