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1
The separation of glutathione transferase subunits by using reverse-phase high-pressure liquid chromatography.利用反相高压液相色谱法分离谷胱甘肽转移酶亚基。
Biochem J. 1987 Jul 15;245(2):423-8. doi: 10.1042/bj2450423.
2
Single-step purification and h.p.l.c. analysis of glutathione transferase 8-8 in rat tissues.大鼠组织中谷胱甘肽转移酶8-8的一步纯化及高效液相色谱分析
Biochem J. 1989 Jun 15;260(3):785-8. doi: 10.1042/bj2600785.
3
Tissue distribution of enzymic methylation of glutathione S-transferase and its effects on catalytic activity. Methylation of glutathione S-transferase 11-11 inhibits conjugating activity towards 1-chloro-2,4-dinitrobenzene.谷胱甘肽S-转移酶的酶促甲基化的组织分布及其对催化活性的影响。谷胱甘肽S-转移酶11-11的甲基化抑制其对1-氯-2,4-二硝基苯的结合活性。
Biochem J. 1992 Feb 15;282 ( Pt 1)(Pt 1):279-89. doi: 10.1042/bj2820279.
4
Hepatic glutathione S-transferases in mice fed on a diet containing the anticarcinogenic antioxidant butylated hydroxyanisole. Isolation of mouse glutathione S-transferase heterodimers by gradient elution of the glutathione-Sepharose affinity matrix.喂食含抗癌抗氧化剂丁基羟基茴香醚饮食的小鼠肝脏谷胱甘肽S-转移酶。通过谷胱甘肽-琼脂糖亲和基质梯度洗脱分离小鼠谷胱甘肽S-转移酶异二聚体。
Biochem J. 1991 Jul 15;277 ( Pt 2)(Pt 2):501-12. doi: 10.1042/bj2770501.
5
Characterization of methylation of rat liver cytosolic glutathione S-transferases by using reverse-phase h.p.l.c. and chromatofocusing.利用反相高效液相色谱法和色谱聚焦法对大鼠肝脏胞质谷胱甘肽S-转移酶的甲基化进行表征。
Biochem J. 1990 Sep 1;270(2):483-9. doi: 10.1042/bj2700483.
6
An improved method for the separation and quantification of glutathione S-transferase subunits in rat tissue using high-performance liquid chromatography.一种使用高效液相色谱法分离和定量大鼠组织中谷胱甘肽S-转移酶亚基的改进方法。
J Chromatogr. 1989 Jul 19;474(2):435-40. doi: 10.1016/s0021-9673(01)93940-8.
7
Selective elution of rodent glutathione S-transferases and glyoxalase I from the S-hexyglutathione-Sepharose affinity matrix.从S-己基谷胱甘肽-琼脂糖亲和基质中选择性洗脱啮齿动物谷胱甘肽S-转移酶和乙二醛酶I 。
Biochem J. 1988 Nov 1;255(3):913-22. doi: 10.1042/bj2550913.
8
Polymorphism of the glutathione transferase subunit 3 in Sprague-Dawley rats involves a reactive cysteine residue.斯普拉格-道利大鼠谷胱甘肽转移酶亚基3的多态性涉及一个反应性半胱氨酸残基。
Biochem J. 2000 Sep 1;350 Pt 2(Pt 2):405-12.
9
Coupled affinity-reversed-phase high-performance liquid chromatography systems for the measurement of glutathione S-transferases in human tissues.用于测定人体组织中谷胱甘肽S-转移酶的偶联亲和-反相高效液相色谱系统
J Chromatogr A. 1994 Jul 29;676(1):65-79. doi: 10.1016/0021-9673(94)80456-7.
10
Purification and characterization of glutathione S-transferases P, S and N. Isolation from rat liver of Yb1 Yn protein, the existence of which was predicted by subunit hybridization in vitro.谷胱甘肽S-转移酶P、S和N的纯化与特性分析。从大鼠肝脏中分离出Yb1 Yn蛋白，其存在是通过体外亚基杂交预测的。
Biochem J. 1984 Dec 15;224(3):839-52. doi: 10.1042/bj2240839.

引用本文的文献

1
Nuclear shield: a multi-enzyme task-force for nucleus protection.核保护伞：保护细胞核的多酶任务组。
PLoS One. 2010 Dec 10;5(12):e14125. doi: 10.1371/journal.pone.0014125.
2
Exposure to chlorinated acetic acids: Responses of peroxidase and glutathione S-transferase activity in pine needles.接触氯化乙酸：松针过氧化物酶和谷胱甘肽 S-转移酶活性的响应。
Environ Sci Pollut Res Int. 1997;4(3):163-71. doi: 10.1007/BF02986326.
3
Reaction of detoxification mechanisms in suspension cultured spruce cells (Picea abies L. Karst.) to heavy metals in pure mixture and in soil eluates.悬浮培养的云杉细胞（欧洲云杉）对纯混合物和土壤洗脱液中重金属的解毒机制反应
Environ Sci Pollut Res Int. 2003;10(4):225-34. doi: 10.1065/espr2002.10.138.
4
Polymorphic glutathione S-transferase subunit 3 of rat liver exhibits different susceptibilities to carbon tetrachloride: differences in their interactions with heat-shock protein 90.大鼠肝脏的多态性谷胱甘肽S-转移酶亚基3对四氯化碳表现出不同的敏感性：它们与热休克蛋白90相互作用的差异。
Biochem J. 2003 Jun 1;372(Pt 2):611-6. doi: 10.1042/BJ20021788.
5
Polymorphism of the glutathione transferase subunit 3 in Sprague-Dawley rats involves a reactive cysteine residue.斯普拉格-道利大鼠谷胱甘肽转移酶亚基3的多态性涉及一个反应性半胱氨酸残基。
Biochem J. 2000 Sep 1;350 Pt 2(Pt 2):405-12.
6
Altered glutathione transferase levels in rat skin inflamed due to contact hypersensitivity: induction of the alpha-class subunit 1.接触性超敏反应所致大鼠皮肤炎症中谷胱甘肽转移酶水平的改变：α类亚基1的诱导
Biochem J. 1998 Nov 1;335 ( Pt 3)(Pt 3):605-10. doi: 10.1042/bj3350605.
7
Glutathione S-transferases of the yeast Yarrowia lipolytica have unusually large molecular mass.解脂耶氏酵母的谷胱甘肽S-转移酶具有异常大的分子量。
Biochem J. 1998 Aug 1;333 ( Pt 3)(Pt 3):839-45. doi: 10.1042/bj3330839.
8
Isolation and characterization of glutathione S-transferase isozymes from sorghum.高粱谷胱甘肽S-转移酶同工酶的分离与鉴定
Plant Physiol. 1998 Jul;117(3):877-92. doi: 10.1104/pp.117.3.877.
9
Growth hormone- and testosterone-dependent regulation of glutathione transferase subunit A5 in rat liver.生长激素和睾酮对大鼠肝脏谷胱甘肽转移酶亚基A5的依赖性调节
Biochem J. 1998 Jun 15;332 ( Pt 3)(Pt 3):763-8. doi: 10.1042/bj3320763.
10
The role of human glutathione transferases and epoxide hydrolases in the metabolism of xenobiotics.人类谷胱甘肽转移酶和环氧化物水解酶在异生素代谢中的作用。
Environ Health Perspect. 1997 Jun;105 Suppl 4(Suppl 4):791-9. doi: 10.1289/ehp.105-1470052.

本文引用的文献

1
The influence of soluble binding proteins on lipophile transport and metabolism in hepatocytes.可溶性结合蛋白对肝细胞中亲脂性物质转运和代谢的影响。
Biochem J. 1981 May 1;195(2):441-52. doi: 10.1042/bj1950441.
2
Evidence that the Ya and Yc subunits of glutathione transferase B (ligandin) are the products of separate genes.谷胱甘肽转移酶B（配体结合蛋白）的Ya和Yc亚基是不同基因产物的证据。
Eur J Biochem. 1982 Sep 1;126(3):459-63. doi: 10.1111/j.1432-1033.1982.tb06802.x.
3
Evidence that the Yb subunits of hepatic glutathione transferases represent two different but related families of polypeptides.肝脏谷胱甘肽转移酶的Yb亚基代表两个不同但相关的多肽家族的证据。
Eur J Biochem. 1983 Dec 1;137(1-2):125-9. doi: 10.1111/j.1432-1033.1983.tb07805.x.
4
Purification and characterization of glutathione S-transferases P, S and N. Isolation from rat liver of Yb1 Yn protein, the existence of which was predicted by subunit hybridization in vitro.谷胱甘肽S-转移酶P、S和N的纯化与特性分析。从大鼠肝脏中分离出Yb1 Yn蛋白，其存在是通过体外亚基杂交预测的。
Biochem J. 1984 Dec 15;224(3):839-52. doi: 10.1042/bj2240839.
5
Glutathione transferases: nomenclature.谷胱甘肽转移酶：命名法
Biochem Pharmacol. 1984 Aug 15;33(16):2539-40. doi: 10.1016/0006-2952(84)90621-x.
6
Rat liver glutathione S-transferases. Complete nucleotide sequence of a glutathione S-transferase mRNA and the regulation of the Ya, Yb, and Yc mRNAs by 3-methylcholanthrene and phenobarbital.大鼠肝脏谷胱甘肽S-转移酶。谷胱甘肽S-转移酶mRNA的完整核苷酸序列以及3-甲基胆蒽和苯巴比妥对Ya、Yb和Yc mRNA的调控。
J Biol Chem. 1984 Apr 25;259(8):5182-8.
7
The nucleotide sequence of a rat liver glutathione S-transferase subunit cDNA clone.大鼠肝脏谷胱甘肽S-转移酶亚基cDNA克隆的核苷酸序列。
J Biol Chem. 1984 May 10;259(9):5536-42.
8
Conversion of cholesterol to androgens by rat testes: comparison of interstitial cells and seminiferous tubules.大鼠睾丸将胆固醇转化为雄激素的研究：间质细胞与曲细精管的比较。
Endocrinology. 1969 Mar;84(3):488-96. doi: 10.1210/endo-84-3-488.
9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
Glutathione transferase from rat testis.来自大鼠睾丸的谷胱甘肽转移酶。
Methods Enzymol. 1985;113:507-10. doi: 10.1016/s0076-6879(85)13067-3.

利用反相高压液相色谱法分离谷胱甘肽转移酶亚基。

The separation of glutathione transferase subunits by using reverse-phase high-pressure liquid chromatography.

作者信息

Ostlund Farrants A K, Meyer D J, Coles B, Southan C, Aitken A, Johnson P J, Ketterer B

机构信息

Department of Biochemistry, Middlesex Hospital Medical School, London, U.K.

出版信息

Biochem J. 1987 Jul 15;245(2):423-8. doi: 10.1042/bj2450423.

DOI:10.1042/bj2450423
PMID:3663168
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1148139/
Abstract

A simple method is described for the separation and quantification of the subunits of GSH transferases present in rat tissue extracts. This method, involving GSH-agarose affinity chromatography followed by reverse-phase h.p.l.c., is rapid and sufficiently sensitive to measure 5 micrograms of each subunit in a mixture. Examples are given of its application to extracts of rat kidney, adrenal, testicular interstitial cells and seminiferous tubules. The analysis of seminiferous tubules indicates that the technique may be of value for the identification of novel subunits. Preliminary separations of subunits from human GSH transferases are also described.

摘要

本文描述了一种简单的方法，用于分离和定量大鼠组织提取物中谷胱甘肽转移酶（GSH transferases）的亚基。该方法包括谷胱甘肽琼脂糖亲和色谱，随后进行反相高效液相色谱，快速且灵敏度足以测量混合物中每个亚基5微克的含量。文中给出了该方法应用于大鼠肾脏、肾上腺、睾丸间质细胞和生精小管提取物的示例。生精小管的分析表明，该技术可能对鉴定新亚基有价值。文中还描述了从人谷胱甘肽转移酶中初步分离亚基的情况。