去甲肾上腺素灌注豚鼠中功能性解偶联蛋白的诱导。对分离的褐色脂肪细胞的研究。
Induction of functional uncoupling protein in guinea pigs infused with noradrenaline. Studies with isolated brown adipocytes.
作者信息
Cunningham S A, Nicholls D G
机构信息
Department of Biochemistry, University of Dundee, Scotland, U.K.
出版信息
Biochem J. 1987 Jul 15;245(2):485-91. doi: 10.1042/bj2450485.
Abstract
Continuous infusion of noradrenaline over the interscapular brown fat of guinea pigs maintained at thermoneutrality (28-32 degrees C) induces changes similar to those after cold-adaptation. (1) Multilocular fat droplets appear within the brown adipocytes. (2) The number of mitochondria per adipocyte and the total number of adipocytes both increase. (3) Noradrenaline addition to isolated adipocytes causes near maximal uncontrolled respiration. (4) The cells become more sensitive to fatty acid-induced uncoupling. (5) The tissue-specific uncoupling protein per mg of mitochondrial protein is increased 5-fold. Specific alpha- and beta-agonists were also chronically infused. (6) Separate infusion of phenylephrine or isoprenaline was not able to stimulate mitochondriogenesis or hyperplasia. (7) Adipocytes from these animals could not be uncoupled by acute noradrenaline. (8) Simultaneous chronic infusion of phenylephrine and isoprenaline reproduced the effects of chronic noradrenaline infusion.
摘要
在处于热中性（28 - 32摄氏度）的豚鼠肩胛间棕色脂肪上持续输注去甲肾上腺素会引发与冷适应后相似的变化。（1）棕色脂肪细胞内出现多泡脂肪滴。（2）每个脂肪细胞的线粒体数量以及脂肪细胞的总数均增加。（3）向分离的脂肪细胞中添加去甲肾上腺素会导致接近最大程度的不受控制的呼吸作用。（4）细胞对脂肪酸诱导的解偶联变得更加敏感。（5）每毫克线粒体蛋白中组织特异性解偶联蛋白增加了5倍。还长期输注了特异性α和β激动剂。（6）单独输注去氧肾上腺素或异丙肾上腺素无法刺激线粒体生成或增生。（7）这些动物的脂肪细胞不能被急性去甲肾上腺素解偶联。（8）同时长期输注去氧肾上腺素和异丙肾上腺素重现了长期输注去甲肾上腺素的效果。
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