白细胞介素-27 抑制鲤鱼春季病毒血症病毒在斑马鱼中的复制。
IL-27 suppresses spring viremia of carp virus replication in zebrafish.
机构信息
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266200, China.
出版信息
Fish Shellfish Immunol. 2023 Feb;133:108530. doi: 10.1016/j.fsi.2023.108530. Epub 2023 Jan 9.
Interleukin (IL) 27 is a member of the IL-12 family and is a heterodimeric cytokine composed of IL-27A and Epstein-Barr virus-induced 3 (EBI3). It plays an important role in regulating inflammation and cancer progression. IL-27A not only functions by dimerizing with EBI3 but also acts alone. Here, we report that IL-27A and EBI3 suppress spring viremia of carp virus (SVCV) replication in zebrafish. Expression analysis reveals that il-27a and ebi3 were significantly upregulated in the ZF4 cells by SVCV and poly(I:C), and in the zebrafish caudal fin (ZFIN) cells overexpressed with SVCV genes. Interestingly, il-27a and ebi3 were not modulated by IFNφ1, indicating that they are not IFN stimulated genes (ISGs). Furthermore, overexpression of IL-27A and EBI3 alone inhibited SVCV replication in the EPC cells, but less potent than co-expression of IL-27A and EBI3. Intriguingly, IL-27A could not induce the expression of irf3, ifn, isg15 and mx1. Taken together, our results demonstrate that IL-27A and EBI3 activate innate antiviral response in an IFN independent manner in zebrafish.
白细胞介素 (IL) 27 是 IL-12 家族的一员，是由 IL-27A 和 Epstein-Barr 病毒诱导的 3 (EBI3) 组成的异二聚体细胞因子。它在调节炎症和癌症进展方面起着重要作用。IL-27A 不仅通过与 EBI3 二聚化起作用，而且还可以单独起作用。在这里，我们报告 IL-27A 和 EBI3 抑制草鱼白细胞增生症病毒 (SVCV) 在斑马鱼中的复制。表达分析显示，SVCV 和多聚 (I:C) 可显著上调 ZF4 细胞中的 il-27a 和 ebi3，而过表达 SVCV 基因的斑马鱼尾鳍 (ZFIN) 细胞中也显著上调 il-27a 和 ebi3。有趣的是，IFNφ1 并未调节 il-27a 和 ebi3，表明它们不是 IFN 刺激基因 (ISGs)。此外，单独过表达 IL-27A 和 EBI3 可抑制 EPC 细胞中的 SVCV 复制，但与 IL-27A 和 EBI3 的共表达相比效果较弱。有趣的是，IL-27A 不能诱导 irf3、ifn、isg15 和 mx1 的表达。总之，我们的结果表明，IL-27A 和 EBI3 在斑马鱼中以 IFN 非依赖的方式激活先天抗病毒反应。
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