Zhu Tao, Cheng Yan-Ting, Ma Yan-Zhu, Zhao Shuai, Li Xia
Second Clinical College of Shanxi University of CM, Jinzhong 030619, China.
Zhongguo Zhen Jiu. 2023 Jan 1;43(1):67-71. doi: 10.13703/j.0255-2930.20220527-0001.
To observe the effect of wheat-grain moxibustion at "Dazhui" (GV 14), "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) on Wnt/β-catenin signaling pathway in bone marrow cell in mice with bone marrow inhibition, and to explore the possible mechanism of wheat-grain moxibustion in treating bone marrow inhibition.
Forty-five SPF male CD1(ICR) mice were randomly divided into a blank group, a model group and a wheat-grain moxibustion group, 15 mice in each group. The bone marrow inhibition model was established by intraperitoneal injection of 80 mg/kg of cyclophosphamide (CTX). The mice in the wheat-grain moxibustion group were treated with wheat-grain moxibustion at "Dazhui" (GV 14), "Zusanli" (ST 36) and "Sanyinjiao" (SP 6), 3 moxa cones per acupoint, 30 s per moxa cone, once a day, for 7 consecutive days. The white blood cell count (WBC) was measured before modeling, before intervention and 3, 5 d and 7 d into intervention. After intervention, the general situation of mice was observed; the number of nucleated cells in bone marrow was detected; the serum levels of interleukin-3 (IL-3), interleukin-6 (IL-6) and granulocyte macrophage colony stimulating factor (GM-CSF) were measured by ELISA; the protein and mRNA expression of β-catenin, cyclinD1 and C-Myc in bone marrow cells was measured by Western blot and real-time PCR method.
Compared with the blank group, the mice in the model group showed sluggish reaction, unstable gait, decreased body weight, and the WBC, number of nucleated cells in bone marrow as well as serum levels of IL-3, IL-6, GM-CSF were decreased (<0.01), and the protein and mRNA expression of β-catenin, cyclinD1 and C-Myc was decreased (<0.01). Compared with the model group, the mice in the wheat-grain moxibustion group showed better general condition, and WBC, the number of nucleated cells in bone marrow as well as serum levels of IL-3, IL-6, GM-CSF were increased (<0.01, <0.05), and the protein and mRNA expression of β-catenin, cyclinD1 and C-Myc was increased (<0.05).
Wheat-grain moxibustion shows therapeutic effect on bone marrow inhibition, and its mechanism may be related to activating Wnt/β-catenin signaling pathway in bone marrow cells, improving bone medullary hematopoiesis microenvironment and promoting bone marrow cell proliferation.
观察麦粒灸“大椎”（GV 14）、“足三里”（ST 36）及“三阴交”（SP 6）对骨髓抑制小鼠骨髓细胞Wnt/β-连环蛋白信号通路的影响，探讨麦粒灸治疗骨髓抑制的可能机制。
将45只SPF级雄性CD1（ICR）小鼠随机分为空白组、模型组和麦粒灸组，每组15只。通过腹腔注射80 mg/kg环磷酰胺（CTX）建立骨髓抑制模型。麦粒灸组小鼠采用麦粒灸“大椎”（GV 14）、“足三里”（ST 36）及“三阴交”（SP 6），每穴3壮，每壮灸30 s，每天1次，连续7天。分别于造模前、干预前及干预后3、5、7 d检测白细胞计数（WBC）。干预后，观察小鼠一般情况；检测骨髓有核细胞数量；采用酶联免疫吸附测定法（ELISA）检测血清白细胞介素-3（IL-3）、白细胞介素-6（IL-6）及粒细胞巨噬细胞集落刺激因子（GM-CSF）水平；采用蛋白质免疫印迹法（Western blot）及实时荧光定量聚合酶链反应（real-time PCR）法检测骨髓细胞中β-连环蛋白、细胞周期蛋白D1（cyclinD1）及原癌基因C-Myc的蛋白及mRNA表达。
与空白组比较，模型组小鼠反应迟钝，步态不稳，体重下降，WBC、骨髓有核细胞数量及血清IL-3、IL-6、GM-CSF水平降低（<0.01），β-连环蛋白、cyclinD1及C-Myc的蛋白及mRNA表达降低（<0.01）。与模型组比较，麦粒灸组小鼠一般情况较好，WBC、骨髓有核细胞数量及血清IL-3、IL-6、GM-CSF水平升高（<0.01，<0.05），β-连环蛋白、cyclinD1及C-Myc的蛋白及mRNA表达升高（<0.05）。
麦粒灸对骨髓抑制具有治疗作用，其机制可能与激活骨髓细胞Wnt/β-连环蛋白信号通路、改善骨髓造血微环境、促进骨髓细胞增殖有关。
