Chevalier J, Pommier M T, Crémieux A
Laboratoire de Microbiologie, Faculté de Pharmacie, Marseille, France.
Ann Inst Pasteur Microbiol. 1987 Jul-Aug;138(4):427-37. doi: 10.1016/0769-2609(87)90060-3.
This study of 8 strains of cutaneous corynebacteria, including 5 strains of "JK corynebacteria", concerns the fatty acid composition of cells grown in the presence or not of Tween 80, which enhances their growth rate. The fatty acid methyl esters were analysed by gas-liquid chromatography. The fatty acid profile of the 8 strains agreed with the general pattern of the genus Corynebacterium, with major amounts of palmitic acid (C16:0) and oleic acid (C18:1) and with only trace amounts of 10-methyl octodecanoic acid (tuberculostearic acid). Iso and anteiso branched acids were not present. The 5 strains had high levels of stearic acid (C18:0). When cells were grown in the presence of Tween-80, the fatty acid composition was modified for all strains: their profiles showed high levels of oleic acid (C18:1) and smaller quantities of palmitoleic acid (C16:1). These results confirm the advantage of fatty acid composition for identification of Corynebacteria, and particularly for cutaneous strains, and emphasize the influence of the culture medium, especially that of Tween-80.
这项对8株皮肤棒状杆菌（包括5株“JK棒状杆菌”）的研究，关注了在添加或不添加吐温80（吐温80可提高其生长速率）的情况下生长的细胞的脂肪酸组成。通过气液色谱法分析脂肪酸甲酯。这8株菌株的脂肪酸谱与棒状杆菌属的一般模式相符，主要含有大量的棕榈酸（C16:0）和油酸（C18:1），仅含有微量的10-甲基十八烷酸（结核硬脂酸）。不存在异支链酸和anteiso支链酸。这5株菌株的硬脂酸（C18:0）含量较高。当细胞在吐温80存在的情况下生长时，所有菌株的脂肪酸组成均发生改变：它们的图谱显示油酸（C18:1）含量较高，而棕榈油酸（C16:1）含量较少。这些结果证实了脂肪酸组成在棒状杆菌鉴定中的优势，尤其是对皮肤菌株的鉴定，并强调了培养基的影响，特别是吐温80的影响。
