Corina D L, Sesardic D
J Gen Microbiol. 1980 Jan;116(1):61-8. doi: 10.1099/00221287-116-1-61.
Gas-liquid chromatography (g.l.c.) was investigated as a potential tool in the classification and identification of Cornyebacterium strains isolated from human skin, on the basis of the g.l.c. profile of the trimethylsilyl derivatives of their mycolic acid methyl esters. The g.l.c. patterns of five skin corynebacteria were compared with those of reference strains Corynebacterium diphtheriae PW8 and Corynebacterium xerosis NCTC 9755 and NCTC 7929. Further compositional information was obtained by gas-liquid chromatography/mass spectrometry (g.l.c./m.s.) of mycolates from C. diphtheriae PW8 and two of the skin isolates. In addition to identifying and examining the individual mycolate species and comparing the differences in mycolate profile between skin corynebacteria and between reference strains, a limited assessment was made of the possibility of distinguishing between organisms at both strain and species levels on the basis of mycolic acid composition as revealed by g.l.c. "fingerprinting".
基于分枝菌酸甲酯的三甲基硅烷基衍生物的气相色谱（g.l.c.）图谱，研究了气相色谱法作为从人皮肤分离的棒状杆菌菌株分类和鉴定的潜在工具。将五种皮肤棒状杆菌的g.l.c.模式与参考菌株白喉棒状杆菌PW8以及干燥棒状杆菌NCTC 9755和NCTC 7929的模式进行了比较。通过对白喉棒状杆菌PW8和两种皮肤分离株的霉菌酸进行气相色谱/质谱联用（g.l.c./m.s.）获得了进一步的组成信息。除了鉴定和检查各个霉菌酸种类并比较皮肤棒状杆菌之间以及参考菌株之间霉菌酸谱的差异外，还基于g.l.c. “指纹图谱”揭示的分枝菌酸组成，对在菌株和物种水平区分生物体的可能性进行了有限的评估。
