Yun Hui, Liang Bin, He Zhangwei, Li Minghan, Zong Simin, Wang Zhenfei, Ge Bin, Zhang Peng, Li Xiangkai, Wang Aijie
Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key Laboratory for Resources Utilization Technology of Unconventional Water of Gansu Province, Gansu Academy of Membrane Science and Technology, Lanzhou, 730020, China; Gansu Key Laboratory of Biomonitoring and Bioremediation for Environment Pollution, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China.
State Key Laboratory of Urban Water Resource and Environment, School of Civil & Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, China.
J Environ Manage. 2023 Apr 1;331:117278. doi: 10.1016/j.jenvman.2023.117278. Epub 2023 Jan 11.
Methane production through anaerobic digestion (AD) of municipal sludge is economic and eco-friendly, which is commonly affected by temperature and pollutants residues. However, little is known about methanogenesis in psychrophilic AD (PAD) with temperature variations that simulating seasonal variations and with antibiotic stress. Here, two groups of AD systems with oxytetracycline (OTC) were operated with temperature maintained at 35 °C and 15 °C or variation to explore the influence to methanogenesis. The acetic acid was noticeably accumulated in OTC groups initially (P < 0.001). Methane production was noticeably inhibited initially in PAD with OTC, but had been stimulated later at 35 °C. The dominant acetoclastic methanogens Methanosaeta gradually decreased to 15.48% and was replaced by methylotrophic Methanomethylovorans (73.43%) in PAD with OTC. Correspondingly, the abundances of functional genes related to methylotrophic methanogenesis were also higher in these groups. Besides, genes involving in methane oxidation had over 50 times higher abundances in PAD with OTC groups in the second phase. Further investigation is essential to understand the main dynamics of methanogenesis in PAD and to clear the related molecular mechanism for future methane production regulation in sludge systems.
通过城市污泥厌氧消化（AD）产生甲烷既经济又环保，但其通常受温度和污染物残留的影响。然而，对于模拟季节变化的温度变化以及抗生素胁迫下嗜冷厌氧消化（PAD）中的产甲烷作用，人们了解甚少。在此，两组添加土霉素（OTC）的厌氧消化系统分别在35℃和15℃恒温或变温条件下运行，以探究其对产甲烷作用的影响。最初，OTC组中乙酸明显积累（P < 0.001）。添加OTC的PAD中，甲烷产量最初受到明显抑制，但随后在35℃时得到刺激。在添加OTC的PAD中，主要的乙酸营养型产甲烷菌甲烷八叠球菌逐渐降至15.48%，并被甲基营养型的嗜甲基甲烷菌（73.43%）取代。相应地，这些组中与甲基营养型产甲烷作用相关的功能基因丰度也更高。此外，在第二阶段，添加OTC的PAD组中参与甲烷氧化的基因丰度高出50多倍。进一步研究对于了解PAD中产甲烷作用的主要动态以及阐明污泥系统未来甲烷产量调控的相关分子机制至关重要。
