全反式维甲酸抑制大鼠和人类小肠黏膜中酰基辅酶A：视黄醇酰基转移酶（EC 2.3.1.76）介导的视黄醇酯化作用。

All-trans-retinoic acid inhibits retinol esterification by acyl-CoA: retinol acyltransferase (EC 2.3.1.76) from rat and human small intestinal mucosa.

Müller H, Norum K R

Institute for Nutrition Research, University of Oslo, Norway.

Br J Nutr. 1986 Jan;55(1):37-41. doi: 10.1079/bjn19860007.

Abstract

Formation of retinyl esters catalyzed by acyl-CoA:retinol acyltransferase (ARAT; retinol fatty-acyltransferase; EC 2.3.1.76) from intestinal mucosa has been studied in vitro in the presence of all-trans-retinoic acid. 2. The incubation system contained microsomal preparations from the tissue tested, radioactive retinol and palmitoyl-CoA. The product formed was separated from the substrates by chromatography on alumina columns. 3. All-trans-retinoic acid inhibited ARAT both from rat and human intestinal mucosa. 4. Inhibition occurred instantly. At a concentration of retinol of 80 microM, a 50% inhibition was obtained with 50 microM-retinoic acid. 5. The inhibition of ARAT by retinoic acid may be of importance for normal retinol absorption in patients receiving retinoid therapy.

摘要

在全反式视黄酸存在的情况下，已在体外研究了由肠黏膜中的酰基辅酶A：视黄醇酰基转移酶（ARAT；视黄醇脂肪酰基转移酶；EC 2.3.1.76）催化形成视黄酯的过程。2. 孵育体系包含来自受试组织的微粒体制剂、放射性视黄醇和棕榈酰辅酶A。通过在氧化铝柱上进行色谱分离，将形成的产物与底物分离。3. 全反式视黄酸抑制大鼠和人肠黏膜中的ARAT。4. 抑制作用即刻发生。在视黄醇浓度为80微摩尔时，50微摩尔视黄酸可产生50%的抑制作用。5. 视黄酸对ARAT的抑制作用对于接受类视黄醇治疗的患者正常吸收视黄醇可能具有重要意义。