Yu Fangming, Liang Xin, Li Yanying, Su Yanlan, Tang Shuting, Wei Jiayu, Liu Kehui, Ma Jiangming, Li Yi
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, China.
Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, Guangxi Normal University, Guilin, 541004, China.
Environ Sci Pollut Res Int. 2023 Mar;30(14):41766-41781. doi: 10.1007/s11356-023-25216-0. Epub 2023 Jan 13.
In the present study, a modified silicon adsorbent (MDSA) was used as a passivator, and we explored the mechanism by which the MDSA helps B. pilosa L. alleviate Cd-induced oxidative stress and its effect on the rhizosphere microbial community. Therefore, a field study was conducted, and MDSA was applied at four levels (control (0 mg m), A1 (100 mg m), A2 (200 mg m), and A3 (400 mg m)). The application of MDSA significantly increased the soil pH and decreased the acid-soluble Cd content, which decreased by 30.3% with A3 addition. The addition of MDSA increased the relative abundance of Sordariomycetes due to the increased invertase activity and total nitrogen (TN) and total phosphorus (TP) contents, and the increased soil pH led to increased relative abundances of Alphaproteobacteria and Thermoleophilia. Meanwhile, MDSA addition significantly decreased the Cd concentrations in leaves and stems, which decreased by 19.7 to 39.5% in stems and 24.6 to 43.2% in leaves. All MDSA additions significantly decreased the translocation factor (TF) values of Cd, which decreased by 30.5% (A1), 50.9% (A2), and 52.7% (A3). Moreover, peroxidase (POD) from the antioxidant enzyme system and glutathione (GSH) from the nonenzymatic system played vital roles in scavenging reactive oxygen intermediates (ROIs) such as HO and ⋅O in leaves, thereby helping B. pilosa L. alleviate Cd-induced oxidative stress and promote plant growth. Hence, our study indicated that MDSA application improved the rhizosphere soil environment, reconstructed the soil microbial community, helped B. pilosa L. alleviate Cd-induced oxidative stress, and promoted plant growth.
在本研究中，一种改性硅吸附剂（MDSA）被用作钝化剂，我们探究了MDSA帮助白花鬼针草缓解镉诱导的氧化应激的机制及其对根际微生物群落的影响。因此，进行了一项田间试验，以四个水平施用MDSA（对照（0 mg m）、A1（100 mg m）、A2（200 mg m）和A3（400 mg m））。MDSA的施用显著提高了土壤pH值，降低了酸溶性镉含量，添加A3时酸溶性镉含量降低了30.3%。由于蔗糖酶活性、总氮（TN）和总磷（TP）含量增加，MDSA的添加提高了粪壳菌纲的相对丰度，土壤pH值升高导致α-变形菌纲和嗜热放线菌纲的相对丰度增加。同时，添加MDSA显著降低了叶片和茎中的镉浓度，茎中镉浓度降低了19.7%至39.5%，叶片中镉浓度降低了24.6%至43.2%。所有MDSA添加处理均显著降低了镉的转运系数（TF）值，A1降低了30.5%，A2降低了50.9%，A3降低了52.7%。此外，抗氧化酶系统中的过氧化物酶（POD）和非酶系统中的谷胱甘肽（GSH）在清除叶片中诸如HO和·O等活性氧中间体（ROIs）方面发挥了重要作用，从而帮助白花鬼针草缓解镉诱导的氧化应激并促进植物生长。因此，我们的研究表明，施用MDSA改善了根际土壤环境，重建了土壤微生物群落，帮助白花鬼针草缓解镉诱导的氧化应激，并促进了植物生长。
