College of Environment and Resources, Guangxi Normal University, 541004, Guilin, China.
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, China; College of Life Science, Guangxi Normal University, 541004, Guilin, China; Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, Guangxi Normal University, 541004, Guilin, China.
Chemosphere. 2022 May;295:133943. doi: 10.1016/j.chemosphere.2022.133943. Epub 2022 Feb 9.
In the present study, field experiments were conducted in Side village, Yangshuo, Guilin, Guangxi Province, China, using four C-BPA application levels (control (0 mg m), T1 (100 mg m), T2 (200 mg m) and T3 (400 mg m)) to clarify the mechanism by which a chitosan-based phosphorus adsorbent (C-BPA) applied as a passivator helps Bidens pilosa L. (B. pilosa L.) alleviate cadmium (Cd)-induced oxidative stress in Cd-contaminated soil. In the aqueous phase, C-BPA successfully adsorbed Cd on the surface primarily via ion exchange, and C-BPA has potential Cd adsorption capacity, enabling its use as a passivator in real Cd-contaminated environments. In Cd-contaminated soils, under C-BPA application at the T3 level, the pH value increased by 11.2%, and the acid-soluble form of Cd decreased by 26.5%. Additionally, the application of C-BPA improved the rhizosphere soil environment and impacted the soil microbial community diversity and structure. Among soil microbes, the soil fungal community was more sensitive than bacteria to C-BPA application. Dehydrogenase, acetic acid, soil pH and Eurotiomycetes or Dothideomycetes significantly impacted Cd accumulation in the leaves of B. pilosa L.; Cd accumulation in leaves was decreased by 68.1% under C-BPA application at the T3 level. Additionally, the variation of increased catalase (CAT) and peroxidase (POD) jointly promoted plant growth; the plant weight was increased by 112.7% under the C-BPA application at the T3 level. Notably, the production of CAT and POD by B. pilosa L. was more effective than the synthesis of glutathione (GSH) in helping B. pilosa L. eliminate excess reactive oxygen species (ROS). Therefore, our findings demonstrated that the application of C-BPA to Cd-contaminated soil can greatly improve the rhizosphere soil environment, help B. pilosa L. eliminate ROS and promote plant growth.
在本研究中，在中国广西桂林阳朔县的 Side 村进行了田间试验，使用四种 C-BPA 施用量（对照（0 mg m）、T1（100 mg m）、T2（200 mg m）和 T3（400 mg m））来阐明壳聚糖基磷吸附剂（C-BPA）作为钝化剂如何帮助三叶鬼针草（B. pilosa L.）缓解镉（Cd）污染土壤中 Cd 诱导的氧化应激。在水相中，C-BPA 通过离子交换成功地在表面上吸附 Cd，并且 C-BPA 具有潜在的 Cd 吸附能力，使其能够在实际的 Cd 污染环境中用作钝化剂。在 Cd 污染土壤中，在 T3 水平下施加 C-BPA 时，pH 值增加了 11.2%，可溶酸态 Cd 减少了 26.5%。此外，C-BPA 的应用改善了根际土壤环境，并影响了土壤微生物群落多样性和结构。在土壤微生物中，土壤真菌群落对 C-BPA 的应用比细菌更敏感。脱氢酶、乙酸、土壤 pH 值和 Eurotiomycetes 或 Dothideomycetes 显著影响三叶鬼针草叶片中 Cd 的积累；在 T3 水平下施加 C-BPA 可使 Cd 积累减少 68.1%。此外，CAT 和 POD 活性的增加共同促进了植物生长；在 T3 水平下施加 C-BPA 可使植物重量增加 112.7%。值得注意的是，三叶鬼针草 L. 产生的 CAT 和 POD 比合成谷胱甘肽（GSH）更有效地帮助三叶鬼针草 L. 消除过量的活性氧（ROS）。因此，我们的研究结果表明，将 C-BPA 施用于 Cd 污染土壤可以极大地改善根际土壤环境，帮助三叶鬼针草 L. 消除 ROS 并促进植物生长。
