College of Basic Medicine, Putian University, Putian 351100, Fujian, PR China; Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, Fujian, PR China; Putian University Key Laboratory of Translational Tumor Medicine in Fujian Province, Putian 351100, Fujian, China, PR.
College of Pharmaceutical and Medical Technology, Putian University, Putian 351100, Fujian, PR China.
Int J Biol Macromol. 2023 Apr 1;233:123223. doi: 10.1016/j.ijbiomac.2023.123223. Epub 2023 Jan 11.
Tailoring magnetic nanocarriers with tunable properties is of great significance for the development of multifunctional candidate materials in numerous fields. Herein, we report a one-pot biomimetic silicification-based method for the synthesis of silica-coated magnetic nanoparticles. The synthesis process was mild, low cost, and highly efficient, which took only about 21 min compared with 4.5-120 h in other literature. Then, the carriers had been characterized by VSM, SEM, TEM, XRD, FT-IR, and EDS to confirm their function. To evaluate the usefulness of the carriers, they were adopted to couple the purification and immobilization of β-1,3-xylanase from the cell lysate in a single step with high immobilization yield (92.8 %) and high activity recovery (82.4 %). The immobilized enzyme also retained 58.4 % of the initial activity after 10 cycles and displayed good storage properties, and improved thermal stability, which would be promising in algae biomass bioconversion as well as other diverse applications.
定制具有可调特性的磁性纳米载体对于开发众多领域的多功能候选材料具有重要意义。在此，我们报告了一种基于仿生硅化的一锅法合成硅涂层磁性纳米粒子的方法。与其他文献中 4.5-120 小时相比，该合成过程温和、成本低、效率高，仅需约 21 分钟。然后，通过 VSM、SEM、TEM、XRD、FT-IR 和 EDS 对载体进行了表征，以确认其功能。为了评估载体的有用性，采用它们在一步中实现从细胞裂解物中纯化和固定β-1,3-木聚糖酶，固定化产率高（92.8%），酶活回收率高（82.4%）。固定化酶在 10 次循环后仍保留初始活性的 58.4%，并且具有良好的储存稳定性和提高的热稳定性，在藻类生物质生物转化以及其他各种应用中具有广阔的应用前景。
