Biochemical Engineering Research Center, Anhui University of Technology, Ma'anshan, Anhui 243002, PR China; School of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui 243002, PR China; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, PR China.
Biochemical Engineering Research Center, Anhui University of Technology, Ma'anshan, Anhui 243002, PR China; School of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui 243002, PR China.
Int J Biol Macromol. 2023 Mar 1;230:123249. doi: 10.1016/j.ijbiomac.2023.123249. Epub 2023 Jan 11.
To obtain lignin from lignocellulosic biomass, phenoxyethanol (EPH) was employed to construct a biphasic solvent system. The concentration of EPH in this biphasic solvent system was first studied to determine a pretreatment condition for fractionation of lignin. Then, the fractionation of lignin from rice straw was performed under the conditions of temperature 130 °C, cooking time 60 min and sulfuric acid concentration 0.1 M, in 70 % aqueous EPH solvent system. The results showed that 50.97 %, 49.52 % or 82.02 % of the removed lignin with the purity of 89.04 %, 91.30 % or 84.76 % was regenerated from EPH liquor using dimethyl carbonate (DMC), dimethoxymethane (DMM) or diethyl ether (DE) as precipitant, respectively. Additionally, the weight-average molecular weight (Mw) and dispersity index (Đ) of the regenerated lignin decreased to 4247-4809 g/mol and 1.26-1.60 compared with that of the original lignin (5654 g/mol and 4.78). Finally, the compositional and structural characteristics of lignin, e.g., molecular weight and molecular structure, were also investigated by DSC, HSQC and elemental analysis.
为了从木质纤维素生物质中获得木质素，采用苯氧乙醇（EPH）构建了双相溶剂体系。首先研究了该双相溶剂体系中 EPH 的浓度，以确定用于木质素分级的预处理条件。然后，在 70%的 EPH 水溶液溶剂体系中，在温度 130°C、蒸煮时间 60 分钟和硫酸浓度 0.1M 的条件下，从稻草中分级分离木质素。结果表明，分别用碳酸二甲酯（DMC）、二甲氧基甲烷（DMM）或二乙醚（DE）作为沉淀剂，从 EPH 溶液中再生出的木质素的去除率为 50.97%、49.52%或 82.02%，纯度为 89.04%、91.30%或 84.76%。此外，与原木质素（5654g/mol，Đ=4.78）相比，再生木质素的重均分子量（Mw）和分散指数（Đ）降低至 4247-4809g/mol 和 1.26-1.60。最后，通过 DSC、HSQC 和元素分析等方法，还研究了木质素的组成和结构特性，如分子量和分子结构。
