Oliver Cristian, Céspedes Constanza, Santibañez Natacha, Ruiz Pamela, Romero Alex
Laboratorio de Inmunología y Estrés de Organismos Acuáticos, Facultad de Ciencias Veterinarias, Instituto de Patología Animal, Universidad Austral de Chile, Valdivia, Chile.
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Talcahuano, Chile.
J Fish Dis. 2023 May;46(5):591-596. doi: 10.1111/jfd.13757. Epub 2023 Jan 14.
Public health is facing a new challenge due to the increased bacterial resistance to most of the conventional antibacterial agents. Inadequate use of antibiotics in the Chilean aquaculture industry leads to the generation of multidrug resistance bacteria. Many fish pathogenic bacteria produce biofilm upon various sources of stress such as antibiotics, which provides several survival advantages for the bacterial life in community and can constitute a reservoir of pathogens in the marine environment. Being florfenicol a broad-spectrum antibiotic commonly used to treat infections in aquaculture, the aim of this study was to assess whether this antibiotic modulates in vitro the biofilm formation in several isolates of Piscirickettsia salmonis. Standard antibiotic-micro broth 96-flat well plates were used to determinate the minimal inhibitory concentration of florfenicol in eight different P. salmonis isolates. In vitro findings, with P. salmonis growing in the presence and absence of the antibiotic, exhibited a statistically significantly increase (p < .05) in biofilm formation in all the bacterial isolates cultivated with sub-MIC (defined as the half of the minimal inhibitory concentration in the presence of antibiotic) of florfenicol compared with controls (antibiotic-free broth). In conclusion, sub-MIC of florfenicol induced an increased biofilm formation in all P. salmonis isolates tested.
由于细菌对大多数传统抗菌药物的耐药性增加，公共卫生正面临新的挑战。智利水产养殖业中抗生素使用不当导致多重耐药菌的产生。许多鱼类病原菌在抗生素等各种应激源作用下会形成生物膜，这为细菌群体生活提供了多种生存优势，并且可能成为海洋环境中病原体的储存库。氟苯尼考是水产养殖中常用的一种广谱抗生素，本研究的目的是评估这种抗生素是否在体外调节鲑鱼立克次氏体几种分离株的生物膜形成。使用标准抗生素微量肉汤96孔平板来测定氟苯尼考在八种不同鲑鱼立克次氏体分离株中的最小抑菌浓度。体外研究结果显示，在有抗生素和无抗生素条件下生长的鲑鱼立克次氏体，与对照组（无抗生素肉汤）相比，在用氟苯尼考亚抑菌浓度（定义为存在抗生素时最小抑菌浓度的一半）培养的所有细菌分离株中，生物膜形成在统计学上有显著增加（p < 0.05）。总之，氟苯尼考亚抑菌浓度诱导了所有测试的鲑鱼立克次氏体分离株生物膜形成增加。
