有机磷酸酯对人工湿地微生物群落和蛋白质组学的影响及其去除机制
Effect of organophosphate esters on microbial community and proteomics in constructed wetlands and its removal mechanism.
作者信息
Lu Shaoyong, Zou Tao, Qin Pan, Zhang Xuan, Wang Guoqiang, Qin Yuchun, Wang Quan
机构信息
State Environmental Protection Scientific Observation and Research Station for Lake Dongtinghu (SEPSORSLD), National Engineering Laboratory for Lake Pollution Control and Ecological Restoration, State Key Laboratory of Environmental Criteria and Risk Assessment, Research Centre of Lake Environment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
College of Ecological Environment, Hainan Tropical Ocean University, Sanya, 572022, China.
出版信息
Chemosphere. 2023 Apr;319:137803. doi: 10.1016/j.chemosphere.2023.137803. Epub 2023 Jan 12.
Organophosphorus esters (OPEs) may cause potential risks to human health and ecological environment safety when they enter water. In this study, A lab-scale vertical flow constructed wetlands (VFCWs) exposed to dichlorvos (DDVP) was carried out to reveal removal effect and mechanism of DDVP in VFCWs. The findings indicated CWs can remove DDVP with 94%-95% average removal efficiency in long-term operation. Besides, soluble phosphate (SRP) and total phosphorus (TP) were removed by VFCWs with 48.60%-64.10% average removal efficiency, and the addition of DDVP enhanced the effect of CWs on nitrogen removal. High-throughput sequencing demonstrated that Massiilia (2.67%-18.06%), Denitratisoma (2.07%-3.80%) and SM1A02 (2.85%-4.67%) played an important role in the removal process of DDVP in VFCWs. Proteomics of plant root showed that A0A3B6ARQ4 (increased 29.53 times) and LOC_Os11g38050.1 protein (decreased 0.001 times) responsed more sensitive to OPEs which suggested DDVP enhancesenhancing plants energy production and hydrolyase synthesis, thereby promoting it hydrolysis. Therefore, the use of VFCWs to treat organic phosphate wastewater has future prospects.
有机磷酸酯（OPEs）进入水体后可能对人类健康和生态环境安全造成潜在风险。本研究通过开展实验室规模的、暴露于敌敌畏（DDVP）的垂直流人工湿地（VFCWs）实验，以揭示VFCWs中DDVP的去除效果及机制。研究结果表明，在长期运行中，人工湿地对DDVP的平均去除效率可达94%-95%。此外，VFCWs对可溶性磷酸盐（SRP）和总磷（TP）的平均去除效率为48.60%-64.10%，添加DDVP增强了人工湿地对氮的去除效果。高通量测序表明，马赛菌属（2.67%-18.06%）、脱氮索菌属（2.07%-3.80%）和SM1A02（2.85%-4.67%）在VFCWs中DDVP的去除过程中发挥了重要作用。植物根系蛋白质组学研究表明，A0A3B6ARQ4（增加了29.53倍）和LOC_Os11g38050.1蛋白（减少了0.001倍）对OPEs反应更敏感，这表明DDVP增强了植物的能量产生和水解酶合成，从而促进其水解。因此，利用VFCWs处理有机磷废水具有广阔的前景。
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