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基于便携式荧光测试条对罐头食品中双酚 A 的选择性和比率荧光传感。

Selective and ratiometric fluorescence sensing of bisphenol A in canned food based on portable fluorescent test strips.

机构信息

Jingdezhen Ceramic University, School of Materials Science and Engineering, Jingdezhen, 333403, PR China.

Jingdezhen Ceramic University, School of Materials Science and Engineering, Jingdezhen, 333403, PR China.

出版信息

Anal Chim Acta. 2023 Feb 1;1240:340728. doi: 10.1016/j.aca.2022.340728. Epub 2022 Dec 19.

Abstract

In this study, a conversion method and molecular imprinting technology were used to design molecularly imprinted polymers (MIP)-based ratiometric fluorescence test papers. The ZnO quantum dots (ZnO QDs) acted as the background quantum dots and ZIF-8 raw material. Carbon dots (CDs) were used as the identification signals. The imprinting layer achieved a selective function. Therefore, a ZnO@ZIF-8/CDs@MIPs sensor was designed for the detection of Bisphenol A (BPA). The sensor exhibited a fast response time for BPA detection. In addition, the sensor demonstrated that effective detection of BPA can still be achieved in complex environments. The detection limit of this sensor was 0.778 nM with a linear range of 0-60 nM. The corresponding test solutions exhibited clear changes from blue to yellow. The selectivity experiments results demonstrated that ZnO@ZIF-8/CDs@MIPs only exhibit excellent selective recognition effect for BPA. ZnO@ZIF-8/CDs@MIPs-2 was used for the detection of BPA in canned food and compared with the results of HPLC detection of BPA. The two spiked recovery ranges were 96.58-102.04% and 97.43-103.82%, respectively. In addition, the prepared ZnO@ZIF-8/CDs@MIPs-2 test paper visually recognized BPA under ultraviolet light. This study provides guidelines for the design and application of fluorescent test papers for quick detection in practical applications.

摘要

在这项研究中,使用转换方法和分子印迹技术设计了基于分子印迹聚合物(MIP)的比率荧光试纸。ZnO 量子点(ZnO QDs)作为背景量子点和 ZIF-8 原料,碳点(CDs)用作识别信号。印迹层实现了选择性功能。因此,设计了一种 ZnO@ZIF-8/CDs@MIPs 传感器用于检测双酚 A(BPA)。该传感器对 BPA 的检测具有快速的响应时间。此外,该传感器表明,即使在复杂环境中,仍能有效地检测 BPA。该传感器的检测限为 0.778 nM,线性范围为 0-60 nM。相应的测试溶液从蓝色变为黄色。选择性实验结果表明,ZnO@ZIF-8/CDs@MIPs 仅对 BPA 表现出优异的选择性识别效果。ZnO@ZIF-8/CDs@MIPs-2 用于检测罐头食品中的 BPA,并与 HPLC 检测 BPA 的结果进行比较。两个加标回收率范围分别为 96.58-102.04%和 97.43-103.82%。此外,制备的 ZnO@ZIF-8/CDs@MIPs-2 试纸在紫外光下可目视识别 BPA。本研究为荧光试纸在实际应用中的快速检测设计和应用提供了指导。

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